Poplawski A M, He C, Irwin J A, Manners J M
Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, Brisbane, Australia.
Curr Genet. 1997 Jul;32(1):66-72. doi: 10.1007/s002940050249.
Previous research has indicated that biotypes A and B of Collectotrichum gloeosporioides that infect Stylosanthes spp. in Australia are asexual and vegetatively incompatible. Selectable marker genes conferring resistance either to hygromycin or phleomycin were introduced into isolates of these biotypes. Vectors conferring resistance to hygromycin and carrying telomeric sequences from Fusarium oxysporum replicated autonomously in C. gloesoporioides and gave frequencies of transformation 100-times higher than vectors that integrated into the genome. Monoconidial colonies resistant to both antibiotics were recovered when hygromycin-resistant biotype-A transformants carrying an autonomously replicating vector were paired in culture with a phleomycin-resistant biotype-B transformant carrying integrative vector sequences. Molecular analysis of double antibiotic-resistant progeny indicated that they contained the autonomous vector in a biotype-B genetic background. Results indicate that transfer of the autonomous vector had occurred from biotype A to biotype B, demonstrating the potential for transfer of genetic information between these biotypes.
先前的研究表明，在澳大利亚感染柱花草属植物的胶孢炭疽菌生物型A和B是无性的且营养体不亲和。将赋予潮霉素或博来霉素抗性的选择标记基因导入这些生物型的分离株中。赋予潮霉素抗性并携带尖孢镰刀菌端粒序列的载体在胶孢炭疽菌中自主复制，其转化频率比整合到基因组中的载体高100倍。当携带自主复制载体的潮霉素抗性生物型A转化体与携带整合载体序列的博来霉素抗性生物型B转化体在培养中配对时，获得了对两种抗生素都有抗性的单分生孢子菌落。对双重抗生素抗性后代的分子分析表明，它们在生物型B的遗传背景中含有自主载体。结果表明，自主载体已从生物型A转移到生物型B，证明了这些生物型之间遗传信息转移的可能性。
