Hoffmann W E, Dorner J L
Am J Vet Res. 1977 Oct;38(10):1553-6.
The serum half-life of various canine alkaline phosphatase isoenzymes has been determined. Isoenzymes can be divided into 2 groups with regard to their half-life. Isoenzymes with a half-life of approximately 3 days include the hepatic alkaline phosphatase and the steroid-induced alkaline phosphatase. Those with a half-life of under 6 minutes include the alkaline phosphatase from placenta, kidney, intestine, and the hepatic and steroid-induced iosenzymes after removal of sialic acid. The inability to demonstrate some of the isoenzymes of alkaline phosphatase in serum of diseases animals is attributed to their short half-life. The relationship of the short half-life to the sialic acid moiety of the protein is discussed.
已测定了各种犬碱性磷酸酶同工酶的血清半衰期。就半衰期而言，同工酶可分为两组。半衰期约为3天的同工酶包括肝碱性磷酸酶和类固醇诱导的碱性磷酸酶。半衰期在6分钟以下的同工酶包括来自胎盘、肾脏、肠道的碱性磷酸酶，以及去除唾液酸后的肝和类固醇诱导的同工酶。在患病动物血清中无法检测到某些碱性磷酸酶同工酶，这归因于它们的半衰期较短。文中讨论了半衰期短与蛋白质唾液酸部分的关系。
