Hartmann C, Landgraf M, Bate M, Jäckle H
Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg, D-37077 Göttingen, Germany.
EMBO J. 1997 Sep 1;16(17):5299-309. doi: 10.1093/emboj/16.17.5299.
Krüppel (Kr) encodes a zinc finger-type transcription factor expressed in spatially and temporally restricted patterns during Drosophila embryogenesis. Molecular targets and the mechanism of Kr action have been studied extensively during the early segmentation process, but only little is known about Kr requirement during later development. We report the identification of a target gene of Kr, termed knockout (ko), isolated by virtue of Krüppel in vitro binding sites. Loss and gain of function experiments show that Kr activity maintains ko expression in a subset of muscles. ko encodes a novel protein expressed in several embryonic tissues including Kr-expressing muscles. Movements of embryos deficient for ko activity are uncoordinated. Their muscle pattern is normal, but the patterns of neuromuscular innervation are specifically disarranged. The results suggest that the Kr target gene ko is required for proper innervation of specific muscles by RP motoneurons.
克鲁佩尔（Kr）编码一种锌指型转录因子，在果蝇胚胎发育过程中以时空受限的模式表达。在早期胚胎分割过程中，对Kr的分子靶点和作用机制进行了广泛研究，但对于其在后期发育中的需求却知之甚少。我们报告了一个名为敲除（ko）的Kr靶基因的鉴定，该基因是通过克鲁佩尔体外结合位点分离得到的。功能缺失和功能获得实验表明，Kr活性维持了ko在一部分肌肉中的表达。ko编码一种在包括表达Kr的肌肉在内的几种胚胎组织中表达的新型蛋白质。缺乏ko活性的胚胎运动不协调。它们的肌肉模式正常，但神经肌肉支配模式却出现了特异性紊乱。结果表明，Kr靶基因ko是运动神经元对特定肌肉进行正确支配所必需的。
