Roth-Maier D A, Hirschvogl G, Eder K, Kirchgessner M
Institut für Ernährungsphysiologie, Technischen Universität München, Freising-Weihenstephan.
Z Ernahrungswiss. 1997 Jun;36(2):176-81. doi: 10.1007/BF01611397.
The present study investigated the effect of various dietary riboflavin supplementations (0 to 4000 mg/kg) during lactation on riboflavin concentrations of liver, carcass (bled body without intestine and liver), and milk in the rat. The experiment was conducted until the 14th day of lactation; milk samples were drawn on the 7th and 13th day of lactation. Riboflavin concentrations of milk raised continuously with increasing riboflavin supplementation; in the range between 0 and 10 mg/kg riboflavin supplementation, there was a linear relationship, and in the range between 12 and 4000 mg/kg there was a logarithmic relationship between riboflavin supplementation and riboflavin concentration in the milk. Maximum riboflavin concentration of milk obtained by supplementation with 4000 mg/kg was twelve-fold higher than without riboflavin supplementation. For riboflavin supplementation up to 12 mg/kg, riboflavin concentrations in milk on the 7th day of lactation and that on the 13th day of lactation were not different. In contrast, in rats fed diets with higher riboflavin supplementation, riboflavin concentrations were higher by 25% in average in milk on the 13th day of lactation than in milk on the 7th day of lactation. Contrary to the milk, riboflavin concentrations in liver and carcass exhibited a saturation, which was achieved at a supplementation of 6 mg/kg (liver) and 10 mg/kg (carcass), respectively. Maximum riboflavin concentrations obtained at a supplementation of 4000 mg/ kg were 1.9- and 2.3-fold higher for liver and carcass, respectively, than concentrations obtained without riboflavin supplementation. The dose-response relationship using riboflavin concentrations of liver and carcass as response factors indicates a riboflavin requirement of 8 to 9 mg/kg for lactating rats fed a semisynthetic diet with 17.4 MJ ME/kg dry matter and 20.8% protein in dry matter.
本研究调查了哺乳期大鼠摄入不同剂量（0至4000毫克/千克）膳食核黄素对其肝脏、胴体（去除肠道和肝脏的放血后的身体）和乳汁中核黄素浓度的影响。实验持续至哺乳期第14天；在哺乳期第7天和第13天采集乳汁样本。乳汁中的核黄素浓度随着核黄素补充量的增加而持续升高；在0至10毫克/千克核黄素补充量范围内，存在线性关系，而在12至4000毫克/千克范围内，核黄素补充量与乳汁中核黄素浓度之间存在对数关系。补充4000毫克/千克核黄素时获得的乳汁中核黄素最大浓度比不补充核黄素时高12倍。对于补充量高达12毫克/千克的核黄素，哺乳期第7天和第13天乳汁中的核黄素浓度没有差异。相比之下，在摄入核黄素补充量较高日粮的大鼠中，哺乳期第13天乳汁中的核黄素浓度平均比哺乳期第7天高25%。与乳汁相反，肝脏和胴体中的核黄素浓度呈现饱和状态，分别在补充6毫克/千克（肝脏）和10毫克/千克（胴体）时达到饱和。补充4000毫克/千克时获得的肝脏和胴体中核黄素最大浓度分别比不补充核黄素时获得的浓度高1.9倍和2.3倍。以肝脏和胴体中的核黄素浓度作为反应因子的剂量反应关系表明，对于摄入每千克干物质含17.4兆焦代谢能和20.8%干物质蛋白质的半合成日粮的哺乳期大鼠，核黄素需求量为8至9毫克/千克。
