通过凝胶包埋抗体过滤法从小鼠羊水纯化甲胎蛋白。

Purification of alpha-fetoprotein from mouse amniotic fluid by gel-entrapped antibody filtration.

作者信息

Mizejewski G J, Simon R, Vonnegut M

出版信息

J Immunol Methods. 1979;31(3-4):333-9. doi: 10.1016/0022-1759(79)90146-7.

Abstract

A one-step immunospecific affinity chromatographic method for purification of mouse alpha-fetoprotein (AFP) directly from amniotic fluid is described. The procedure employed a rabbit anti-AFP immunoglobulin-G entrapped in a polyacrylamide gel matrix prepared as a gel slurry. The presence of impurities could not be demonstrated in the eluted fraction and the recovery of AFP was approximately 26%. Sufficient quantities of AFP were readily purified for raising antibodies and for purity studies.

摘要

本文描述了一种直接从羊水纯化小鼠甲胎蛋白（AFP）的一步免疫特异性亲和色谱法。该方法采用包埋于聚丙烯酰胺凝胶基质（制成凝胶悬液）中的兔抗AFP免疫球蛋白G。洗脱组分中未检测到杂质，AFP的回收率约为26%。可轻松纯化出足够量的AFP用于制备抗体和纯度研究。