de Siqueira A L, Russo M, Steil A A, Facincone S, Mariano M, Jancar S
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Brazil.
J Allergy Clin Immunol. 1997 Sep;100(3):383-8. doi: 10.1016/s0091-6749(97)70253-7.
We have recently described a model of hypersensitivity reaction in the mouse paw, which induces a typical late-phase reaction with a marked eosinophilic infiltrate.
In the search for a murine model of asthma, this model was adapted to the lungs and compared with other models of pulmonary hypersensitivity.
A fragment of heat-coagulated hen's egg white was implanted subcutaneously, and 14 days later, the mice were challenged intratracheally with aggregated ovalbumin. Comparison was made with a group that received subcutaneous injection of soluble ovalbumin in alumen, challenged as described above and with four additional protocols of immunization and challenge.
Forty-eight hours after challenge, the percentage of eosinophils was higher in the egg white implant group (35%) than in the group immunized with ovalbumin in alumen (10.4%). The eosinophil peroxidase activity in lung homogenates of the first group was also significantly higher (529 ng/ml) than that of the second group (43 ng/ml). These results were reproduced in five different mouse strains. Compared with five different models of lung hypersensitivity, the egg white implant model was unique in terms of persistence of the pulmonary eosinophilia. Histopathologic analysis of the lungs of mice immunized with egg white implant showed peribronchial, perivascular, and intraepithelial eosinophil infiltration; morphologic characteristics of bronchoconstriction; and patchy epithelial shedding. At 21 days, in addition to persistence of eosinophil infiltrate, enlarged alveoli, reflecting air trapping, were observed.
On the basis of the characteristics of the model described here, we propose it as a suitable murine model of asthma.
我们最近描述了一种小鼠爪部超敏反应模型，该模型可诱导典型的迟发性反应，并伴有明显的嗜酸性粒细胞浸润。
为寻找哮喘的小鼠模型，对该模型进行肺部适应性改造，并与其他肺部超敏反应模型进行比较。
将热凝固的鸡卵白蛋白片段皮下植入，14天后，用聚集卵清蛋白对小鼠进行气管内激发。与皮下注射可溶性卵清蛋白于内腔、按上述方法激发的一组以及另外四种免疫和激发方案进行比较。
激发后48小时，蛋清植入组嗜酸性粒细胞百分比（35%）高于内腔注射卵清蛋白免疫组（10.4%）。第一组肺匀浆中的嗜酸性粒细胞过氧化物酶活性（529 ng/ml）也显著高于第二组（43 ng/ml）。这些结果在五种不同的小鼠品系中得到重现。与五种不同的肺部超敏反应模型相比，蛋清植入模型在肺部嗜酸性粒细胞增多的持续性方面具有独特性。对蛋清植入免疫小鼠的肺部进行组织病理学分析，显示支气管周围、血管周围和上皮内有嗜酸性粒细胞浸润；有支气管收缩的形态学特征；以及斑片状上皮脱落。在21天时，除嗜酸性粒细胞浸润持续存在外，还观察到肺泡扩大，提示空气潴留。
基于此处所述模型的特征，我们建议将其作为一种合适的哮喘小鼠模型。
