通过高效液相色谱法分离埃德曼降解法释放的氨基酸的苯硫代乙内酰脲衍生物（作者译）
[Separation by high-performance liquid chromatography of phenylthiohydantoin derivatives of amino acids released by Edman degradation (author's transl)].
作者信息
Bollet C, Caude M
出版信息
J Chromatogr. 1976 Jun 23;121(2):323-8. doi: 10.1016/s0021-9673(00)85029-3.
PMID:932149
Abstract
The separation by high-performance liquid chromatography of seventeen phenylthiohydantoin derivatives of amino acids released after Edman degradation of proteins is described. Sixteen phenylthiohydantoin derivatives of amino acids are differentiated within 40 min by liquid-liquid partition on a column packed with Micropak CN (moderately polar alkyl nitrile phase bonded on 10mum porous silica microparticles) and gradient elution with hexane=methylene chloride-isopropanol mixtures.
摘要
本文描述了通过高效液相色谱法对蛋白质经埃德曼降解后释放的17种氨基酸苯硫代乙内酰脲衍生物进行分离的方法。16种氨基酸苯硫代乙内酰脲衍生物在填充有Micropak CN（键合在10μm多孔硅胶微粒上的中等极性烷基腈相）的柱上通过液 - 液分配，并使用己烷 = 二氯甲烷 - 异丙醇混合物进行梯度洗脱，在40分钟内得以区分。
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