苯乙酸、扁桃酸和吡啶羧酸衍生物的反相离子对分配色谱法。

Reversed-phase ion-pair partition chromatography of phenylacetic, mandelic and pyridinecarboxylic acid derivatives.

作者信息

Wahlund K G, Lund U

出版信息

J Chromatogr. 1976 Jul 7;122:269-76. doi: 10.1016/s0021-9673(00)82249-9.

Abstract

Phenylacetic, mandelic and pyridinecarboxylic acids of physiological interest have been separated by ion-pair partition chromatography with 1-pentanol as stationary phase and tetrabutylammonium as counter ion in the aqueous mobile phase. The stability of the chromatographic system is very high and the support is spontaneously re-coated with stationary phase. Untreated plasma samples can be injected in relatively large volumes without serious loss of separating efficiency. A venting system was found efficiently to reduce disturbing early peaks on injection of biological samples.

摘要

具有生理意义的苯乙酸、扁桃酸和吡啶羧酸已通过离子对分配色谱法进行分离，该方法以1-戊醇为固定相，四丁基铵为流动相水溶液中的抗衡离子。色谱系统的稳定性非常高，载体可自发地重新被固定相包覆。未经处理的血浆样品可以较大体积进样，而不会严重损失分离效率。发现一种排气系统能有效减少进样生物样品时出现的干扰性早出峰。