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Simplified handling of high-density genetic filters using rigid plastic laminates.使用刚性塑料层压板简化高密度基因过滤器的处理。
Nucleic Acids Res. 1997 Oct 15;25(20):4160-1. doi: 10.1093/nar/25.20.4160.
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Automated production of high density cosmid and YAC colony filters using a robotic workstation.使用机器人工作站自动生产高密度黏粒和酵母人工染色体菌落滤膜。
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Screening recombinant DNA libraries.
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[Millipore filter: 30 samples at a time].
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Hybridisation techniques on gridded high density DNA and in situ colony filters based on fluorescence detection.基于荧光检测的网格状高密度DNA和原位菌落滤膜杂交技术。
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Effect of membrane dimensions and shear rate on plasma filtration for hollow fibers.
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引用本文的文献

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A contiguous 3-Mb sequence-ready map in the S3-MX region on 21q22.2 based on high- throughput nonisotopic library screenings.基于高通量非同位素文库筛选的21号染色体q22.2区域S3-MX区的一个连续3兆碱基序列就绪图谱。
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A new bacteriophage P1-derived vector for the propagation of large human DNA fragments.一种用于扩增大片段人类DNA的新型噬菌体P1衍生载体。
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An integrated YAC-overlap and 'cosmid-pocket' map of the human chromosome 21.人类21号染色体的YAC重叠与“黏粒库”整合图谱。
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Hybridisation techniques on gridded high density DNA and in situ colony filters based on fluorescence detection.基于荧光检测的网格状高密度DNA和原位菌落滤膜杂交技术。
Nucleic Acids Res. 1994 Aug 25;22(16):3423-4. doi: 10.1093/nar/22.16.3423.
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Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome.小鼠散布重复序列PCR产物的高效高分辨率遗传图谱绘制，迈向小鼠基因组的整合遗传图谱和物理图谱绘制。
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使用刚性塑料层压板简化高密度基因过滤器的处理。

Simplified handling of high-density genetic filters using rigid plastic laminates.

作者信息

Bancroft D R, O'Brien J K, Guerasimova A, Lehrach H

机构信息

Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin (Dahlem), Germany.

出版信息

Nucleic Acids Res. 1997 Oct 15;25(20):4160-1. doi: 10.1093/nar/25.20.4160.

DOI:10.1093/nar/25.20.4160
PMID:9321673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146994/
Abstract

Wet membranes in DNA hybridisation procedures can be very difficult to handle. This adds considerable time to the length of experiments and is a serious obstacle to the performance of large-scale investigations. A method is described to give rigidity to membranes, thereby making them easier to handle and in turn improving both the speed and efficiency of experimental work.

摘要

DNA杂交实验中的湿膜很难处理。这大大延长了实验时间，并且严重阻碍了大规模研究的开展。本文介绍了一种增强膜硬度的方法，从而使其更易于处理，进而提高实验工作的速度和效率。