Nomura M, Ueta Y, Larsen P J, Hannibal J, Serino R, Kabashima N, Shibuya I, Yamashita H
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Endocrinology. 1997 Oct;138(10):4096-100. doi: 10.1210/endo.138.10.5427.
The effect of water deprivation on the expression of pituitary adenylate cyclase-activating polypeptide (PACAP) was examined in the rat subfornical organ (SFO), using a combination of immunohistochemistry and in situ hybridization histochemistry. In the euhydrated condition, PACAP-immunoreactivity (PACAP-IR) and the expression of PACAP gene was observed in the SFO. Water deprivation for 24 h and 48 h caused a significant increase in PACAP gene transcripts in the SFO, compared with euhydrated animals. Additionally, water deprivation for 48 h caused an increase in PACAP-IR. This increase of PACAP-IR was demonstrated in both nerve fibers and cell bodies. High correlation was found between the localization of PACAP-IR cell bodies and PACAP messenger RNA synthesizing cell bodies in the peripheral part of the SFO. These results suggest that PACAP in the SFO may play a role in the humoral and neural changes associated with the regulation of body fluid balance after water deprivation.
采用免疫组织化学和原位杂交组织化学相结合的方法，研究了缺水对大鼠穹窿下器（SFO）中垂体腺苷酸环化酶激活多肽（PACAP）表达的影响。在正常水合状态下，在SFO中观察到PACAP免疫反应性（PACAP-IR）和PACAP基因的表达。与正常水合的动物相比，缺水24小时和48小时导致SFO中PACAP基因转录本显著增加。此外，缺水48小时导致PACAP-IR增加。这种PACAP-IR的增加在神经纤维和细胞体中均有表现。在SFO外周部分，PACAP-IR细胞体的定位与PACAP信使核糖核酸合成细胞体之间存在高度相关性。这些结果表明，SFO中的PACAP可能在缺水后与体液平衡调节相关的体液和神经变化中发挥作用。
