Williams R O, Mahaguna V, Sriwongjanya M
Pharmaceutics Division, College of Pharmacy, University of Texas, Austin 78712-1074, USA.
J Chromatogr B Biomed Sci Appl. 1997 Aug 29;696(2):303-6. doi: 10.1016/s0378-4347(97)00235-1.
A high-performance liquid chromatographic method for the determination of diazolidinyl urea (DU) in a cream formulation is described. The aqueous phase of the emulsion was separated by centrifugation, removed, filtered, diluted and applied onto the HPLC system. DU was detected by ultraviolet absorption at a wavelength of 214 nm. The calibration curve was linear over the range of 79-553 micrograms/ml, and identical when determined on consecutive days. The relative standard deviation for repeat determinations was less than 0.5%. Recoveries were 97.74-101.72%. This analytical method is useful for quantitation of DU in cream formulations.
描述了一种用于测定乳膏制剂中重氮烷基脲（DU）的高效液相色谱法。通过离心分离乳液的水相，去除、过滤、稀释后注入高效液相色谱系统。在214nm波长处通过紫外吸收检测DU。校准曲线在79 - 553微克/毫升范围内呈线性，连续几天测定时结果相同。重复测定的相对标准偏差小于0.5%。回收率为97.74 - 101.72%。该分析方法可用于定量乳膏制剂中的DU。
