阴沟肠杆菌对无机磷酸盐趋化缺陷突变体的分离与鉴定
Isolation and characterization of Enterobacter cloacae mutants which are defective in chemotaxis toward inorganic phosphate.
作者信息
Kusaka K, Shibata K, Kuroda A, Kato J, Ohtake H
机构信息
Department of Fermentation Technology, Hiroshima University, Higashi-Hiroshima, Japan.
出版信息
J Bacteriol. 1997 Oct;179(19):6192-5. doi: 10.1128/jb.179.19.6192-6195.1997.
Abstract
Enterobacter cloacae IFO3320 is attracted to Pi when cells are starved for Pi. Two Tn1737KH-induced mutants, which were constitutive for alkaline phosphatase, failed to exhibit Pi taxis even under conditions of Pi limitation. Both of the mutant strains exhibited normal chemotactic responses to peptone, suggesting that they are specifically defective in Pi taxis. Cloning and sequence analysis showed that the TN1737KH insertions were located in either the pstA or pstB genes which encode the channel-forming proteins of the Pi-specific transport (Pst) system in E. cloacae. These results suggest that the E. cloacae Pst system is required for Pi chemoreception.
摘要
阴沟肠杆菌IFO3320在细胞缺乏磷时会被磷吸引。两个由Tn1737KH诱导产生的碱性磷酸酶组成型突变体，即使在磷限制条件下也未能表现出磷趋化性。这两个突变菌株对蛋白胨均表现出正常的趋化反应，表明它们在磷趋化性方面存在特异性缺陷。克隆和序列分析表明，TN1737KH插入位于阴沟肠杆菌中编码磷特异性转运（Pst）系统通道形成蛋白的pstA或pstB基因中。这些结果表明，阴沟肠杆菌的Pst系统是磷化学感受所必需的。
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