铜绿假单胞菌中一个参与磷酸趋化性负调控的基因的克隆与特性分析
Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis.
作者信息
Kato J, Sakai Y, Nikata T, Ohtake H
机构信息
Department of Fermentation Technology, Hiroshima University, Japan.
出版信息
J Bacteriol. 1994 Sep;176(18):5874-7. doi: 10.1128/jb.176.18.5874-5877.1994.
Abstract
Pseudomonas aeruginosa PAO1 exhibited a positive chemotactic response to P(i). The chemotactic response was induced by P(i) limitation. An alkaline phosphatase (AP) constitutive mutant showed a chemotactic response to P(i), regardless of whether the cells were starved for P(i). Sequence analysis and complementation studies showed that the P. aeruginosa phoU gene was involved both in the regulation of AP expression and in the induction of P(i) taxis. However, unlike AP expression, P(i) taxis was not regulated by the phoB gene product.
摘要
铜绿假单胞菌PAO1对无机磷（Pi）表现出正向趋化反应。这种趋化反应是由Pi限制诱导的。一个碱性磷酸酶（AP）组成型突变体对Pi表现出趋化反应，无论细胞是否处于Pi饥饿状态。序列分析和互补研究表明，铜绿假单胞菌的phoU基因既参与AP表达的调控，也参与Pi趋化的诱导。然而，与AP表达不同，Pi趋化不受phoB基因产物的调控。
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