使用蔗糖作为不稳定的绵羊红细胞补体成分——中间复合物EAC142的冷冻保护剂。

The use of sucrose as a cryoprotective agent for the labile sheep erythrocyte-complement component--intermediate complex, EAC142.

作者信息

Hoffmann E M, Brogden M C

出版信息

J Immunol Methods. 1976 Mar;10(2-3):293-9. doi: 10.1016/0022-1759(76)90180-0.

Abstract

Sucrose (15% w/v) was found to be a useful agent for cryopreservation of sheep erythrocytes in the intermediate state EAC142. The ordinarilly very labile SAC142, associated with the cells, did not decay to any appreciable degree during periods of storage of 1, 72 or 132 days at -65 to -70 degrees C. Recovery of erythrocytes after freezing and thawing varied. Our best recovery was 76.6% and the poorest was 43.0%. The poorest recoveries were obtained during the latter part of the 132 day experiment after the freezer malfunctioned on day 35 causing a temperature rise to -50 degrees C which lasted for 3 days. The average spontaneous lysis of cells before freezing and thawing was 5.6%. The average spontaneous lysis for cells which had been stored in the frozen state was 6.9%.

摘要

已发现蔗糖（15% w/v）是用于将处于中间状态EAC142的绵羊红细胞进行冷冻保存的有效试剂。通常非常不稳定的与细胞相关的SAC142，在-65至-70摄氏度下储存1天、72天或132天期间，并未出现明显降解。冻融后红细胞的回收率各不相同。我们的最佳回收率为76.6%，最差的为43.0%。在132天实验的后期，即冷冻机在第35天出现故障导致温度升至-50摄氏度并持续3天之后，获得了最差的回收率。冻融前细胞的平均自发裂解率为5.6%。处于冷冻状态储存的细胞的平均自发裂解率为6.9%。