Fluorometric assay for the determination of DNA-DNA cross-links utilizing Hoechst 33258 at neutral pH values.

Suppr

超能文献

作者信息

Penketh P G, Shyam K, Sartorelli A C

机构信息

Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

出版信息

Anal Biochem. 1997 Oct 1;252(1):210-3. doi: 10.1006/abio.1997.9996.

DOI:10.1006/abio.1997.9996

PMID:9324963

Abstract

摘要

相似文献

Fluorometric assay for the determination of DNA-DNA cross-links utilizing Hoechst 33258 at neutral pH values.

Anal Biochem. 1997 Oct 1;252(1):210-3. doi: 10.1006/abio.1997.9996.

A direct fluorometric assay for DNA in alkaline sucrose gradients using Hoechst 33258 dye.

Am Biotechnol Lab. 1993 Oct;11(11):50-2.

[Interaction of mono- and bisbenzimidazole analogs of Hoechst-33258 with DNA].

Mol Biol (Mosk). 1997 Mar-Apr;31(2):274-80.

Hoechst 33258 as a pH-sensitive probe to study the interaction of amine oxide surfactants with DNA.

Chembiochem. 2005 Jan;6(1):197-203. doi: 10.1002/cbic.200400196.

Sequence-selective interaction of the minor-groove interstrand cross-linking agent SJG-136 with naked and cellular DNA: footprinting and enzyme inhibition studies.

Biochemistry. 2005 Mar 22;44(11):4135-47. doi: 10.1021/bi0479813.

Synthesis and characterization of DNA containing an N1-2'-deoxyinosine-ethyl-N3-thymidine interstrand cross-link: a structural mimic of the cross-link formed by 1,3-bis-(2-chloroethyl)-1-nitrosourea.

Chem Res Toxicol. 2008 Mar;21(3):686-95. doi: 10.1021/tx700422h. Epub 2008 Feb 8.

Anomalous cross-linking by mechlorethamine of DNA duplexes containing C-C mismatch pairs.

Biochemistry. 1999 Mar 23;38(12):3641-8. doi: 10.1021/bi981935j.

Design of new bidentate ligands constructed of two Hoechst 33258 units for discrimination of the length of two A3T3 binding motifs.

J Org Chem. 2006 Jan 6;71(1):125-34. doi: 10.1021/jo051836t.

Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.

Cold Spring Harb Protoc. 2017 May 1;2017(5):2017/5/pdb.prot093567. doi: 10.1101/pdb.prot093567.

Non-radioisotope labelling of DNA with multifunctional labelling agent.

Nucleic Acids Symp Ser. 1993(29):73-4.

引用本文的文献

pH-dependent general base catalyzed activation rather than isocyanate liberation may explain the superior anticancer efficacy of laromustine compared to related 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine prodrugs.

Chem Biol Drug Des. 2018 Jan;91(1):62-74. doi: 10.1111/cbdd.13057. Epub 2017 Jul 17.

When alcohol is the answer: Trapping, identifying and quantifying simple alkylating species in aqueous environments.

Anal Biochem. 2016 Sep 1;508:34-7. doi: 10.1016/j.ab.2016.04.020. Epub 2016 May 14.

A novel gene delivery composite system based on biodegradable folate-poly (ester amine) polymer and thermosensitive hydrogel for sustained gene release.

Sci Rep. 2016 Feb 17;6:21402. doi: 10.1038/srep21402.

Modification of cellular DNA by synthetic aziridinomitosenes.

Bioorg Med Chem. 2015 Dec 1;23(23):7378-85. doi: 10.1016/j.bmc.2015.10.028. Epub 2015 Oct 21.

Influence of glutathione and glutathione S-transferases on DNA interstrand cross-link formation by 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine, the active anticancer moiety generated by laromustine.

Chem Res Toxicol. 2014 Aug 18;27(8):1440-9. doi: 10.1021/tx500197t. Epub 2014 Jul 17.

Influence of phosphate and phosphoesters on the decomposition pathway of 1,2-bis(methylsulfonyl)-1-(2-chloroethyhydrazine (90CE), the active anticancer moiety generated by Laromustine, KS119, and KS119W.

Chem Res Toxicol. 2014 May 19;27(5):818-33. doi: 10.1021/tx500004y. Epub 2014 Mar 24.

A strategy for selective O(6)-alkylguanine-DNA alkyltransferase depletion under hypoxic conditions.

Chem Biol Drug Des. 2012 Aug;80(2):279-90. doi: 10.1111/j.1747-0285.2012.01401.x. Epub 2012 May 23.

Lethality to leukemia cell lines of DNA interstrand cross-links generated by Cloretazine derived alkylating species.

Leuk Res. 2008 Oct;32(10):1546-53. doi: 10.1016/j.leukres.2008.03.005. Epub 2008 May 13.

1,2-Bis(methylsulfonyl)-1-(2-chloroethyl)-2-[[1-(4-nitrophenyl)ethoxy]carbonyl]hydrazine: an anticancer agent targeting hypoxic cells.

Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9282-7. doi: 10.1073/pnas.0409013102. Epub 2005 Jun 17.

Mitomycin resistance in mammalian cells expressing the bacterial mitomycin C resistance protein MCRA.

Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10489-94. doi: 10.1073/pnas.96.18.10489.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验