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1
When alcohol is the answer: Trapping, identifying and quantifying simple alkylating species in aqueous environments.当答案是酒精时：在水环境中捕获、识别和量化简单烷基化物质。
Anal Biochem. 2016 Sep 1;508:34-7. doi: 10.1016/j.ab.2016.04.020. Epub 2016 May 14.
2
Proceedings: A comparison of chemical and microbiological methods for estimating alkylating agent concentration.论文集：用于估计烷基化剂浓度的化学和微生物学方法的比较
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[Identification of alkylating agents. II. Identification of different classes of alkylating agents by a modification of the color reaction with 4-(4-nitrobenzyl)-pyridine (NBP)].
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1
Antitumor sulfonylhydrazines: design, structure-activity relationships, resistance mechanisms, and strategies for improving therapeutic utility.抗肿瘤磺酰肼类化合物：设计、构效关系、耐药机制及提高治疗效用的策略
J Med Chem. 2015 May 14;58(9):3639-71. doi: 10.1021/jm501459c. Epub 2015 Feb 9.
2
Influence of glutathione and glutathione S-transferases on DNA interstrand cross-link formation by 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine, the active anticancer moiety generated by laromustine.谷胱甘肽和谷胱甘肽S-转移酶对1,2-双（甲基磺酰基）-1-（2-氯乙基）肼（洛莫司汀产生的活性抗癌部分）形成DNA链间交联的影响。
Chem Res Toxicol. 2014 Aug 18;27(8):1440-9. doi: 10.1021/tx500197t. Epub 2014 Jul 17.
3
Influence of phosphate and phosphoesters on the decomposition pathway of 1,2-bis(methylsulfonyl)-1-(2-chloroethyhydrazine (90CE), the active anticancer moiety generated by Laromustine, KS119, and KS119W.磷酸盐和磷酸酯对1,2 - 双（甲基磺酰基）-1 - （2 - 氯乙基）肼（90CE）分解途径的影响，90CE是洛莫司汀、KS119和KS119W产生的活性抗癌部分。
Chem Res Toxicol. 2014 May 19;27(5):818-33. doi: 10.1021/tx500004y. Epub 2014 Mar 24.
4
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Crit Rev Oncol Hematol. 2014 Jan;89(1):43-61. doi: 10.1016/j.critrevonc.2013.07.006. Epub 2013 Aug 20.
5
Balancing repair and tolerance of DNA damage caused by alkylating agents.平衡烷化剂引起的 DNA 损伤的修复和耐受。
Nat Rev Cancer. 2012 Jan 12;12(2):104-20. doi: 10.1038/nrc3185.
6
Comparison of DNA lesions produced by tumor-inhibitory 1,2-bis(sulfonyl)hydrazines and chloroethylnitrosoureas.肿瘤抑制性1,2-双（磺酰基）肼与氯乙基亚硝脲产生的DNA损伤比较。
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7
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Cancer Invest. 1997;15(6):588-98. doi: 10.3109/07357909709047601.
8
Fluorometric assay for the determination of DNA-DNA cross-links utilizing Hoechst 33258 at neutral pH values.
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9
Spectroscopic aqueous-phase assay for alkylating activity suitable for automation or multiwell plate application.适用于自动化或多孔板应用的用于烷基化活性的光谱水相测定法。
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10
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Drug Metab Rev. 1984;15(7):1307-34. doi: 10.3109/03602538409029962.

当答案是酒精时：在水环境中捕获、识别和量化简单烷基化物质。

When alcohol is the answer: Trapping, identifying and quantifying simple alkylating species in aqueous environments.

作者信息

Penketh Philip G, Shyam Krishnamurthy, Baumann Raymond P, Zhu Rui, Ishiguro Kimiko, Sartorelli Alan C, Ratner Elena S

机构信息

Department of Obstetrics, Gynecology & Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520-8063, USA.

Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520-8066, USA.

出版信息

Anal Biochem. 2016 Sep 1;508:34-7. doi: 10.1016/j.ab.2016.04.020. Epub 2016 May 14.

DOI:10.1016/j.ab.2016.04.020
PMID:27188264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4955734/
Abstract

Alkylating agents are a significant class of environmental carcinogens as well as commonly used anticancer therapeutics. Traditional alkylating activity assays have utilized the colorimetric reagent 4-(4-nitrobenzyl)pyridine (4NBP). However, 4NBP based assays have a relatively low sensitivity towards harder, more oxophilic alkylating species and are not well suited for the identification of the trapped alkyl moiety due to adduct instability. Herein we describe a method using water as the trapping agent which permits the trapping of simple alkylating electrophiles with a comparatively wide range of softness/hardness and permits the identification of donated simple alkyl moieties.

摘要

烷基化剂是一类重要的环境致癌物，也是常用的抗癌治疗药物。传统的烷基化活性测定方法使用比色试剂4-(4-硝基苄基)吡啶(4NBP)。然而，基于4NBP的测定方法对更难、更亲氧的烷基化物种的灵敏度相对较低，并且由于加合物的不稳定性，不太适合鉴定捕获的烷基部分。在此，我们描述了一种使用水作为捕获剂的方法，该方法允许捕获具有较宽软度/硬度范围的简单烷基化亲电试剂，并允许鉴定所提供的简单烷基部分。