Harris H E, Liljeström M, Lindroos E, Kleinau S
Department of Medicine, Karolinska Institute, Stockholm, Sweden.
Clin Immunol Immunopathol. 1997 Oct;85(1):40-6. doi: 10.1006/clin.1997.4414.
We have investigated the inflammatory cell infiltrates and adhesion molecule expression in the synovial fluid (SF) and synovial membranes (SM) of rats with homologous collagen-induced arthritis. Immunohistochemical staining revealed that the majority of the cells in the SF were granulocytes, expressing CD11b and CD11c. In SM, the majority of the cells were monocytes/macrophages. CD49d and CD49f were expressed mainly in the erosion zone in SM, and ICAM-1 was expressed in the lining layer, in the capillaries, and in the erosion zone. In SF 7% of the cells were ICAM-1 positive. CD2 was more abundant in SM than in SF. These findings might explain the difference in granulocyte counts between SF and SM. CD49d and CD49f expression might retain lymphocytes and monocytes in SM, while granulocytes not expressing CD49d and CD49f are not retained.
我们研究了同源胶原诱导性关节炎大鼠的滑液（SF）和滑膜（SM）中的炎性细胞浸润及黏附分子表达情况。免疫组织化学染色显示，SF中的大多数细胞为粒细胞，表达CD11b和CD11c。在SM中，大多数细胞为单核细胞/巨噬细胞。CD49d和CD49f主要在SM的侵蚀区表达，而细胞间黏附分子-1（ICAM-1）在衬里层、毛细血管及侵蚀区表达。在SF中，7%的细胞ICAM-1呈阳性。CD2在SM中的表达比在SF中更丰富。这些发现可能解释了SF和SM中粒细胞计数的差异。CD49d和CD49f的表达可能会使淋巴细胞和单核细胞滞留在SM中，而不表达CD49d和CD49f的粒细胞则不会滞留。
