Carol M, Pelegrí C, Castellote C, Franch A, Castell M
Department of Physiology-Division IV, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
Clin Exp Immunol. 2000 Apr;120(1):200-8. doi: 10.1046/j.1365-2249.2000.01194.x.
The aim of this study was to examine leucocyte populations in lymphoid organs during AA and to ascertain the relationship with lesions in synovial joints. Popliteal lymph nodes, spleen and knee synovial membranes were removed from both healthy and AA rats at intervals of 3-4 days over a 3-week period. Cryostat sections were stained with MoAbs directed against lymphocyte and macrophage subpopulations, and studied by image analysis. Throughout the arthritic period, high numbers of ED1+ and ED3+ macrophages were seen in both lymphoid compartments and intercellular adhesion molecule-1 (ICAM-1) expression also increased in some zones of lymph nodes and spleen. The percentages of CD4+ and CD8+ cells rose in the splenic zones studied but fell in the lymph node cortex. Very few natural killer (NK) cells were found in lymphoid tissues, but the number rose after AA induction. In synovia from AA rats, ED2+ macrophages proliferated but alpha/beta T cell infiltration was only occasionally observed, accompanied by ED1+ cells and ICAM-1 expression. In conclusion, synovitis developing after AA induction seems to be caused directly by macrophages and indirectly by lymphocytes placed both in popliteal lymph nodes and spleen.
本研究的目的是检查佐剂性关节炎（AA）期间淋巴器官中的白细胞群体，并确定其与滑膜关节病变的关系。在3周的时间内，每隔3 - 4天从健康大鼠和AA大鼠中取出腘淋巴结、脾脏和膝关节滑膜。冰冻切片用针对淋巴细胞和巨噬细胞亚群的单克隆抗体染色，并通过图像分析进行研究。在整个关节炎期间，在两个淋巴区均可见大量ED1 +和ED3 +巨噬细胞，并且在淋巴结和脾脏的某些区域细胞间黏附分子-1（ICAM-1）表达也增加。在所研究的脾区中，CD4 +和CD8 +细胞的百分比上升，但在淋巴结皮质中下降。在淋巴组织中发现的自然杀伤（NK）细胞很少，但在诱导AA后数量增加。在AA大鼠的滑膜中，ED2 +巨噬细胞增殖，但仅偶尔观察到α/β T细胞浸润，并伴有ED1 +细胞和ICAM-1表达。总之，AA诱导后发生的滑膜炎似乎直接由巨噬细胞引起，间接由腘淋巴结和脾脏中的淋巴细胞引起。
