Fujita T, Takahashi S, Yagihashi A, Jimbow K, Sato N
Division of Plastic and Reconstructive Surgery, Sapporo Medical University School of Medicine, Japan.
Transplantation. 1997 Sep 27;64(6):922-5. doi: 10.1097/00007890-199709270-00023.
We studied the effect of FK506 ointment on rat skin allograft survival.
Lewis rat (RT1(1)) skin allografts were histologically evaluated on day 7 after transplantation to recipient ACI (RT1a) rats. We set histological gradings for rejection as follows: grade 1, intraepidermal blister formation; grade 2, incomplete epidermal separation from the dermis; and grade 3, complete epidermal separation from the epidermis. According to these histological criteria, the immunosuppressive effect of FK506 ointment was assessed.
Our data indicated that all recipient rats without FK506 ointment showed grade 3 rejection on day 7 after transplantation. In contrast, the allografts treated with FK506 ointment showed grade 1 rejection. Furthermore the blood concentration of FK506 was below 0.5 ng/ml, minimizing the systemically adverse effects of FK506.
Thus, the present study suggests that the topical administration of FK506 on the skin allografts may be useful and effective in the suppression of skin allograft rejection.
我们研究了FK506软膏对大鼠皮肤同种异体移植物存活的影响。
在将Lewis大鼠（RT1(1)）皮肤同种异体移植物移植到受体ACI（RT1a）大鼠后第7天，对其进行组织学评估。我们将排斥反应的组织学分级设定如下：1级，表皮内水疱形成；2级，表皮与真皮不完全分离；3级，表皮与表皮完全分离。根据这些组织学标准，评估FK506软膏的免疫抑制作用。
我们的数据表明，所有未使用FK506软膏的受体大鼠在移植后第7天均表现出3级排斥反应。相比之下，用FK506软膏处理的同种异体移植物表现出1级排斥反应。此外，FK506的血药浓度低于0.5 ng/ml，将FK506的全身不良反应降至最低。
因此，本研究表明，在皮肤同种异体移植物上局部应用FK506可能对抑制皮肤同种异体移植物排斥反应有用且有效。
