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1
Menadione-resistant Chinese hamster ovary cells have an increased capacity for glutathione synthesis.对甲萘醌耐药的中国仓鼠卵巢细胞具有增强的谷胱甘肽合成能力。
Br J Cancer. 1997;76(7):870-7. doi: 10.1038/bjc.1997.477.
2
Relationship between the adaptive response to oxidants and stable menadione-resistance in Chinese hamster ovary cell lines.
Carcinogenesis. 1996 Apr;17(4):649-54. doi: 10.1093/carcin/17.4.649.
3
Menadione-resistant Chinese hamster cell variants are cross-resistant to hydrogen peroxide and exhibit stable chromosomal and biochemical alterations.抗甲萘醌的中国仓鼠细胞变体对过氧化氢具有交叉抗性，并表现出稳定的染色体和生化改变。
Mol Cell Biochem. 1992 Dec 16;118(2):181-9. doi: 10.1007/BF00299397.
4
Synthesis of heat shock proteins following oxidative challenge: role of glutathione.氧化应激后热休克蛋白的合成：谷胱甘肽的作用
Int J Hyperthermia. 1994 Jul-Aug;10(4):573-86. doi: 10.3109/02656739409009359.
5
Menadione toxicity in two mouse liver established cell lines having striking genetic differences in quinone reductase activity and glutathione concentrations.两种小鼠肝脏建立的细胞系中维生素K3的毒性，这两种细胞系在醌还原酶活性和谷胱甘肽浓度方面存在显著的遗传差异。
Toxicol Appl Pharmacol. 1993 Sep;122(1):101-7. doi: 10.1006/taap.1993.1177.
6
Alterations in intracellular thiol homeostasis during the metabolism of menadione by isolated rat hepatocytes.分离的大鼠肝细胞在甲萘醌代谢过程中细胞内硫醇稳态的变化。
Arch Biochem Biophys. 1984 Dec;235(2):334-42. doi: 10.1016/0003-9861(84)90206-6.
7
Effect of 1-methyl-2-nitrosoimidazole on intracellular thiols and calcium levels in Chinese hamster ovary cells.1-甲基-2-亚硝基咪唑对中国仓鼠卵巢细胞内硫醇和钙水平的影响。
Biochem Pharmacol. 1991 Nov 6;42(11):2153-61. doi: 10.1016/0006-2952(91)90351-5.
8
Menadione causes increases in the level of glutathione and in the activity of gamma-glutamylcysteine synthetase in cultured Chinese hamster V79 cells.
Toxicology. 1996 Aug 1;112(1):45-55. doi: 10.1016/0300-483x(96)03348-3.
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The influence of depleted glutathione levels on the photodynamic action of zinc phthalocyanine in CHO K1 cells.谷胱甘肽水平降低对锌酞菁在CHO K1细胞中光动力作用的影响。
J Clin Laser Med Surg. 2003 Aug;21(4):185-91. doi: 10.1089/104454703768247747.
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Modulation of cytotoxicity of menadione sodium bisulfite versus leukemia L1210 by the acid-soluble thiol pool.酸溶性硫醇池对亚硫酸氢钠甲萘醌抗白血病L1210细胞毒性的调节作用
Cancer Res. 1985 Nov;45(11 Pt 1):5257-62.

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1
Overexpression of the regulatory subunit of gamma-glutamylcysteine synthetase in HeLa cells increases gamma-glutamylcysteine synthetase activity and confers drug resistance.γ-谷氨酰半胱氨酸合成酶调节亚基在人宫颈癌细胞系（HeLa细胞）中的过表达可增加γ-谷氨酰半胱氨酸合成酶活性并赋予耐药性。
Biochem J. 1999 Feb 1;337 ( Pt 3)(Pt 3):559-66.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
STUDIES ON QUINONE-THIOETHERS. I. MECHANISM OF FORMATION AND PROPERTIES OF THIODIONE.醌硫醚的研究。I. 硫二酮的形成机制及性质
Biochemistry. 1963 May-Jun;2:537-43. doi: 10.1021/bi00903a025.
3
A clinical trial of synkavit in the treatment of carcinoma of the bronchus.维生素K治疗支气管癌的临床试验。
Br J Cancer. 1962 Sep;16(3):387-9. doi: 10.1038/bjc.1962.44.
4
Relationship between the adaptive response to oxidants and stable menadione-resistance in Chinese hamster ovary cell lines.
Carcinogenesis. 1996 Apr;17(4):649-54. doi: 10.1093/carcin/17.4.649.
5
High field proton NMR investigations of the metabolic profiles of multidrug-sensitive and -resistant leukaemic cell lines: evidence for diminished taurine levels in multidrug-resistant cells.多药敏感和耐药白血病细胞系代谢谱的高场质子核磁共振研究：耐药细胞中牛磺酸水平降低的证据。
Free Radic Res Commun. 1993;19(6):355-69. doi: 10.3109/10715769309056526.
6
Status of glutathione and glutathione-metabolizing enzymes in menadione-resistant human cancer cells.耐甲萘醌人癌细胞中谷胱甘肽及谷胱甘肽代谢酶的状态
Biochem Pharmacol. 1994 Jan 20;47(2):421-4. doi: 10.1016/0006-2952(94)90036-1.
7
Phase I study of mitomycin C and menadione in advanced solid tumors.丝裂霉素C与甲萘醌用于晚期实体瘤的I期研究。
Cancer Chemother Pharmacol. 1995;36(4):293-8. doi: 10.1007/BF00689046.
8
Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increases sensitivity to irradiation.人淋巴细胞的谷胱甘肽输出：通过抑制其合成来消耗谷胱甘肽会降低输出并增加对辐射的敏感性。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7492-6. doi: 10.1073/pnas.78.12.7492.
9
The metabolism of menadione (2-methyl-1,4-naphthoquinone) by isolated hepatocytes. A study of the implications of oxidative stress in intact cells.分离的肝细胞对维生素K3（2-甲基-1,4-萘醌）的代谢。完整细胞中氧化应激影响的研究。
J Biol Chem. 1982 Oct 25;257(20):12419-25.
10
Menadione-induced cytotoxicity is associated with protein thiol oxidation and alteration in intracellular Ca2+ homeostasis.甲萘醌诱导的细胞毒性与蛋白质巯基氧化及细胞内钙离子稳态改变有关。
Arch Biochem Biophys. 1984 Dec;235(2):343-50. doi: 10.1016/0003-9861(84)90207-8.

对甲萘醌耐药的中国仓鼠卵巢细胞具有增强的谷胱甘肽合成能力。

Menadione-resistant Chinese hamster ovary cells have an increased capacity for glutathione synthesis.

作者信息

Vallis K A, Reglinski J, Garner M, Bridgeman M M, Wolf C R

机构信息

Imperial Cancer Research Fund Molecular Pharmacology Unit, Biomedical Research Centre, Ninewells Hospital and Medical School, Dundee, UK.

出版信息

Br J Cancer. 1997;76(7):870-7. doi: 10.1038/bjc.1997.477.

DOI:10.1038/bjc.1997.477
PMID:9328145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2228060/
Abstract

A cell line (MRc40) resistant to the model quinone compound, menadione, has been isolated from a parental Chinese hamster ovary cell line (CHO-K1). The known relationship between menadione toxicity and glutathione (GSH) depletion led us to investigate whether the mechanism of resistance of MRc40 was related to alteration in GSH homeostasis. Intracellular concentrations of GSH and cysteine (CySH) were twofold and 3.2-fold greater in MRc40 than in CHO-K1. Following exposure to menadione, GSH and CySH were depleted, but subsequent recovery of thiols was more rapid and of greater magnitude in MRc40 than in CHO-K1. Twelve hours after exposure to menadione, the concentrations of GSH and CySH were 9.7- and 4.2-fold greater in MRc40 than in CHO-K1. Using nuclear magnetic resonance (NMR) spectroscopy, we observed the in situ removal of menadione from cell suspensions of CHO-K1 and MRc40. However, only in CHO-K1 did we observe concomitant depletion of NMR-visible GSH. We conclude that the perturbation of GSH metabolism contributes to the resistant phenotype and is an important characteristic of menadione-resistant CHO cells.

摘要

已从亲本中国仓鼠卵巢细胞系（CHO-K1）中分离出对模型醌类化合物甲萘醌具有抗性的细胞系（MRc40）。甲萘醌毒性与谷胱甘肽（GSH）消耗之间的已知关系促使我们研究MRc40的抗性机制是否与GSH稳态的改变有关。MRc40细胞内GSH和半胱氨酸（CySH）的浓度分别比CHO-K1细胞高两倍和3.2倍。暴露于甲萘醌后，GSH和CySH被消耗，但随后MRc40细胞中硫醇的恢复比CHO-K1细胞更快且幅度更大。暴露于甲萘醌12小时后，MRc40细胞中GSH和CySH的浓度分别比CHO-K1细胞高9.7倍和4.2倍。使用核磁共振（NMR）光谱，我们观察到从CHO-K1和MRc40细胞悬液中原位去除甲萘醌的情况。然而，只有在CHO-K1细胞中我们才观察到NMR可见的GSH同时消耗。我们得出结论，GSH代谢的扰动有助于抗性表型，并且是抗甲萘醌CHO细胞的一个重要特征。