Saha K, McKinley G, Volsky D J
Molecular Virology Laboratory, St. Luke's-Roosevelt Hospital Center, College of Physicians and Surgeons, New York 10019, USA.
J Immunol Methods. 1997 Aug 7;206(1-2):21-3. doi: 10.1016/s0022-1759(97)00080-x.
Herpesvirus saimiri (HVS) can infect and immortalize human T lymphocytes of both CD4- and CD8-positive phenotypes. We have previously shown that infection of peripheral blood lymphocytes (PBL) from AIDS patients with HVS predominantly yielded immortalized CD8-positive T cell clones. Here we show that CD4-positive T cells from AIDS patients can be efficiently immortalized by HVS if patient PBL are enriched for CD4-positive T cell subpopulation prior to HVS infection. Such cells can be cloned and maintained in culture for prolonged times, and they exhibit activated T cell phenotype of Th1 class and are susceptible to HIV-1 infection. Several immortalized T cell clones obtained from one out of three AIDS patients tested here were HIV-1 positive and produced infectious virus. This approach permits efficient generation of multiple CD4-positive T cell clones from AIDS patients for functional and virological studies.
猴疱疹病毒（HVS）可感染CD4和CD8阳性表型的人T淋巴细胞并使其永生化。我们之前已经表明，用HVS感染艾滋病患者的外周血淋巴细胞（PBL）主要产生永生化的CD8阳性T细胞克隆。在此我们表明，如果在HVS感染之前将患者的PBL富集CD4阳性T细胞亚群，艾滋病患者的CD4阳性T细胞可被HVS有效地永生化。此类细胞可被克隆并在培养中长时间维持，并且它们表现出Th1类活化T细胞表型，并且易受HIV-1感染。从这里测试的三名艾滋病患者中的一名获得的几个永生化T细胞克隆是HIV-1阳性的，并产生有传染性的病毒。这种方法允许从艾滋病患者有效地产生多个CD4阳性T细胞克隆用于功能和病毒学研究。
