Kang Q, An Y H, Friedman R J
Department of Orthopedic Surgery, Medical University of South Carolina, Charleston 29425, USA.
Am J Vet Res. 1997 Oct;58(10):1171-3.
To assess the effects of multiple freezing-thawing cycles on ultimate indentation load and stiffness of bovine tibial cancellous bone with regard to freezing conditions (saline solution or air) or methods of thawing (saline solution or air).
4 tibias from 4 adult cows.
The proximal portions of the tibias were sectioned to produce 20-mm-thick bone slices. Slices were subjected to 5 freezing-thawing cycles under 4 conditions: freezing with and without saline solution, then thawing in saline solution or exposed to the air. The mechanical properties of the bone slices before and after the treatments were measured, using an indentation test for comparison. Indentation testing was performed before and after freezing-thawing cycles to measure differences in mechanical parameters.
Significant differences in mechanical parameters were not found for bone specimens frozen in saline solution and thawed in saline solution; frozen without saline solution and thawed in air; frozen in saline solution and thawed in air; or frozen without saline solution and thawed in saline solution.
Multiple thawing-freezing cycles do not significantly affect the ultimate indentation load and stiffness of bovine tibial cancellous bone.
评估多次冻融循环对牛胫骨松质骨最终压痕负荷和刚度的影响，涉及冷冻条件（盐溶液或空气）或解冻方法（盐溶液或空气）。
取自4头成年母牛的4根胫骨。
将胫骨近端切成20毫米厚的骨片。骨片在4种条件下进行5次冻融循环：用盐溶液冷冻和不用盐溶液冷冻，然后在盐溶液中解冻或暴露于空气中。使用压痕试验比较处理前后骨片的力学性能。在冻融循环前后进行压痕试验以测量力学参数的差异。
在盐溶液中冷冻并在盐溶液中解冻的骨标本；不用盐溶液冷冻并在空气中解冻的骨标本；在盐溶液中冷冻并在空气中解冻的骨标本；或不用盐溶液冷冻并在盐溶液中解冻的骨标本，力学参数均未发现显著差异。
多次冻融循环对牛胫骨松质骨的最终压痕负荷和刚度没有显著影响。
