Agrewala J N, Deacock S, Jurcevic S, Wilkinson R
Tuberculosis and Related Infections Unit, MRC Clinical Sciences Centre, London, United Kingdom.
Hum Immunol. 1997 Jun;55(1):34-8. doi: 10.1016/s0198-8859(97)00056-6.
The HLA class II isotype and allelic restrictions of peptide recognition were analyzed with T cells from a DRB11501/DRB10901 heterozygous donor. Nineteen T cell clones, all directed against the single mycobacterial epitope p21-40 were tested with HLA homozygous lymphoblastoid cell lines as antigen-presenting cells. The most striking finding has been, that several DR isotype restricted clones recognized the peptide in the context of both parental, but not of unrelated alleles. In contrast, DQ and DP restricted clones responded in the context of one parental allele only. Most DR promiscuous clones produced interferon-gamma but not IL-4, whereas most DQ and DP clones produced IL-4. We postulate that the confinement of DR promiscuity only to the parental alleles was established possibly during thymic maturation of T cells and that the proportions between monogamous and promiscuous T cells may play a role in the MHC mediated influences on host resistance to infections and other immune responses.
利用来自DRB11501/DRB10901杂合供体的T细胞，分析了肽识别的HLA II类同种型和等位基因限制。使用HLA纯合淋巴母细胞系作为抗原呈递细胞，对所有针对单个分枝杆菌表位p21-40的19个T细胞克隆进行了检测。最显著的发现是，几个DR同种型限制的克隆在两个亲本等位基因的背景下都能识别该肽，但在无关等位基因的背景下则不能。相反，DQ和DP限制的克隆仅在一个亲本等位基因的背景下产生反应。大多数DR混杂克隆产生γ干扰素但不产生IL-4，而大多数DQ和DP克隆产生IL-4。我们推测，DR混杂性仅局限于亲本等位基因可能是在T细胞的胸腺成熟过程中建立的，并且一夫一妻制和混杂性T细胞之间的比例可能在MHC介导的对宿主抗感染和其他免疫反应的影响中发挥作用。
