Ellexson M, Lai-Kwan P, Lau M, Muto K, Terasaki P, Cole J, Thompson C, Hildebrand W
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Hum Immunol. 1997 Jun;55(1):66-73. doi: 10.1016/s0198-8859(97)00055-4.
Genetic exchanges often muddle the typing of HLA class I molecules, this is also the case for HLA-B1304. Serologic and molecular DNA class I typing report a B15/B55 type for cell 847, whereas DNA sequencing finds B5501/B1304. HLA-B1304 differs by no more than four amino acids from other HLA-B13 molecules, a comparative analysis of the B13 and B15 families was therefore performed to determine why serologic and molecular DNA approaches report a B15 type for B1304. Comparisons demonstrate that limited differences individuate the B15 and B13 molecules such that the genetic recombination of codons 145 and 163 in the class I heavy chain's alpha 2 alpha helix prompt B1304 to exhibit a B15X21 pattern of serologic cross-reactivity. Molecular DNA class I typing approaches are also swayed by genetic recombinations to type B1304 as a B15 molecule: B15-like nucleotide sequences encoding residues 114, 116, and 145, lead B1304 to exhibit a B15 PCR amplification pattern. Thus, genetic exchanges encoding key amino acids in the class I heavy chain lead molecular and serologic typing approaches to categorize HLA-B*1304 as a member of the B15 family.
基因交换常常使HLA I类分子的分型变得复杂，HLA - B1304的情况也是如此。血清学和分子DNA I类分型报告细胞847为B15/B55型，而DNA测序发现是B5501/B1304。HLA - B1304与其他HLA - B13分子的氨基酸差异不超过四个，因此对B13和B15家族进行了比较分析，以确定为什么血清学和分子DNA方法将B1304报告为B15型。比较表明，有限的差异区分了B15和B13分子，使得I类重链α2α螺旋中密码子145和163的基因重组促使B1304表现出B15X21血清学交叉反应模式。分子DNA I类分型方法也受到基因重组的影响，将B1304分型为B15分子：编码第114、116和145位残基的类似B15的核苷酸序列，导致B1304表现出B15 PCR扩增模式。因此，I类重链中编码关键氨基酸的基因交换导致分子和血清学分型方法将HLA - B*1304归类为B15家族的成员。
