Díaz-Véliz G, Alarcón T, Espinoza C, Dussaubat N, Mora S
Unidad de Farmacología, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Pharmacol Biochem Behav. 1997 Nov;58(3):637-42. doi: 10.1016/s0091-3057(97)90004-6.
The influence of gender, estrous cycle, ovariectomy and ovarian hormones on the behavioral effects of the 5-HT2 receptor antagonist, ketanserin (KET), was studied. Intact males, female rats in the four stages of the estrous cycle and ovariectomized (OVX) female rats 14 days after surgery were used. The OVX rats received progesterone [PROG, 25 mg/kg, subcutaneously (SC)] and/or estradiol benzoate (EB, 10 micrograms/kg, SC). KET (3 mg/kg, SC) was injected 30 min before testing. All the animals were subjected to the following behavioral tests: exploration of an elevated plus-maze and retention of a passive-avoidance response. KET enhanced the exploration of the open arms in diestrous female rats but inhibited this behavior during the other stages of the cycle and in OVX rats injected either with oil or EB. This dose of KET was ineffective in males and in OVX rats injected with PROG. Furthermore, KET inhibited the retention of the passive avoidance response in males, in diestrous and metestrous female rats and in OVX rats injected with oil. In estrous females and in OVX rats injected with EB, KET enhanced the passive-avoidance response. These results demonstrate that the sensitivity to KET differs with the gender, estrous cycle and hormonal treatment and suggest that central serotonergic activity is influenced by the hormonal status of the animal.
研究了性别、发情周期、卵巢切除及卵巢激素对5-羟色胺2（5-HT2）受体拮抗剂酮色林（KET）行为效应的影响。实验使用了成年雄性大鼠、处于发情周期四个阶段的雌性大鼠以及术后14天的去卵巢（OVX）雌性大鼠。OVX大鼠皮下注射孕酮[PROG，25mg/kg]和/或苯甲酸雌二醇（EB，10μg/kg）。测试前30分钟皮下注射KET（3mg/kg）。所有动物均接受以下行为测试：高架十字迷宫探索试验和被动回避反应保持试验。KET增强了处于动情后期的雌性大鼠对开放臂的探索，但在发情周期的其他阶段以及注射油剂或EB的OVX大鼠中抑制了这种行为。该剂量的KET对雄性大鼠以及注射PROG的OVX大鼠无效。此外，KET抑制了雄性大鼠、处于动情后期和动情间期的雌性大鼠以及注射油剂的OVX大鼠的被动回避反应保持。在发情期雌性大鼠以及注射EB的OVX大鼠中，KET增强了被动回避反应。这些结果表明，对KET的敏感性因性别、发情周期和激素处理而异，并提示中枢5-羟色胺能活性受动物激素状态的影响。
