Heiss P, Bermatz S, Wehnes H, Heinzmann U, Senekowitsch-Schmidtke R
Nuklearmedizinische Klinik, Technischen Universität München, Germany.
Anticancer Res. 1997 Jul-Aug;17(4B):3145-7.
The ganglioside GD2 is highly expressed on human tumors of neuroectodermal origin. We investigated by scanning electron microscopy the ganglioside GD2 distribution on the surface of spheroids of the neuroblastoma cell line SK-N-LO. About 50% of cells showed GD2 on the plasma membrane and the distribution of GD2 on most of these cells was heterogeneous, with more GD2 at the contact sites of the cells. The binding kinetics of the chimeric anti-GD2 antibody ch14.18 labelled with I-125 on spheroids (average diameter: 450 microns) was determined by gamma counting. Over 4 hours the antibody concentration was raised substantially but up to 24 hours there was only a very slow further increase. A clustered pattern of bound chimeric anti-GD2 antibody ch14.18 was found on a cross-section of the spheroid by autoradiography.
神经节苷脂GD2在神经外胚层起源的人类肿瘤上高度表达。我们通过扫描电子显微镜研究了神经母细胞瘤细胞系SK-N-LO球体表面神经节苷脂GD2的分布。约50%的细胞在质膜上显示有GD2，并且这些细胞中大多数的GD2分布是不均匀的，在细胞的接触部位有更多的GD2。通过γ计数法测定了用I-125标记的嵌合抗GD2抗体ch14.18在球体（平均直径：450微米）上的结合动力学。在4小时内抗体浓度大幅升高，但直到24小时仅有非常缓慢的进一步增加。通过放射自显影在球体的横截面上发现了结合的嵌合抗GD2抗体ch14.18的聚集模式。
