Plappert U G, Stocker B, Fender H, Fliedner T M
Department of Clinical Physiology, Occupational and Social Medicine, University of Ulm, Germany.
Environ Mol Mutagen. 1997;30(2):153-60. doi: 10.1002/(sici)1098-2280(1997)30:2<153::aid-em8>3.0.co;2-o.
The purpose of this study was to examine whether changes in the repair capacity of blood cells could be used as a valuable biomarker for radiation exposure. To characterize the repair kinetics in nonirradiated and irradiated cells we first performed in vitro split dose experiments. DNA damage and DNA repair capacity were analysed using the comet assay. Our results showed that the first in vitro irradiation affects the repair system of the cells, resulting in a decreased repair capacity after the second irradiation. Furthermore, the second irradiation results in a large amount of DNA damage in the blood cells. To test whether the analysis of the DNA repair capacity after in vitro irradiation is also a valuable method for in vivo studies of donors exposed to radiation, we analysed the repair capacity of blood cells of two exposed groups: patients subjected to a radioiodine therapy and chronically irradiated volunteers from the Chernobyl region. Both groups also showed a significantly impaired repair capacity indicating a stress on the hematopoietic system. In addition, in the group of the Ukrainians DNA damage after in vitro irradiation was significantly higher than in a control group. These results lead to the presumption that the repair capacity and the DNA damage after in vitro irradiation might be a very useful biological marker for radiation exposure in population monitoring.
本研究的目的是检验血细胞修复能力的变化是否可作为辐射暴露的一种有价值的生物标志物。为了表征未辐照和辐照细胞中的修复动力学，我们首先进行了体外分次剂量实验。使用彗星试验分析DNA损伤和DNA修复能力。我们的结果表明，首次体外辐照会影响细胞的修复系统，导致第二次辐照后修复能力下降。此外，第二次辐照会导致血细胞中大量DNA损伤。为了测试体外辐照后DNA修复能力的分析是否也是研究受辐射供体体内情况的一种有价值的方法，我们分析了两个受辐照组血细胞的修复能力：接受放射性碘治疗的患者和来自切尔诺贝利地区的长期受辐照志愿者。两组的修复能力也均显著受损，表明造血系统受到了应激。此外，乌克兰人群组体外辐照后的DNA损伤明显高于对照组。这些结果使人们推测，体外辐照后的修复能力和DNA损伤可能是人群监测中辐射暴露的一种非常有用的生物学标志物。
