Ebert E, Werle B, Jülke B, Kopitar-Jerala N, Kos J, Lah T, Abrahamson M, Spiess E, Ebert W
Thoraxklinik Heidelberg-Rohrbach, Heidelberg, Germany.
Adv Exp Med Biol. 1997;421:259-65. doi: 10.1007/978-1-4757-9613-1_34.
In human lung tumor tissue specimen (n = 73) concentrations of stefins A and B were found to be increased 2.0-fold (p < 0.01) and 1.3-fold (p < 0.01), respectively, as compared to matched normal tissue. Stefin A and B concentrations were higher in primary tumors than in secondary tumors, i.e. metastases from other organs to the lung (p < 0.01; p < 0.05, respectively). Cystatin C concentrations were rather low and did not differ between tumor and normal tissue. Both concentrations of stefins did not correlate with TNM stages. Stefin A was higher in squamous cell carcinoma than in adenocarcinoma (p < 0.01), while stefin B did not show such a difference. At investigation of a relationship between survival probability of patients with primary tumors it was found that increased stefin B concentrations and total cysteine-protease-inhibitory activities but not stefin A concentrations were positively correlated with survival probability. It is concluded that stefins A and B are major contributors to the cysteine protease inhibitory activity in primary lung tumors. Stefin B proved to be a prognostic factor, especially in squamous cell carcinoma.
在73份人肺肿瘤组织标本中，与配对的正常组织相比，发现丝抑蛋白A和B的浓度分别增加了2.0倍（p < 0.01）和1.3倍（p < 0.01）。原发性肿瘤中丝抑蛋白A和B的浓度高于继发性肿瘤，即从其他器官转移至肺部的肿瘤（分别为p < 0.01；p < 0.05）。胱抑素C的浓度相当低，肿瘤组织与正常组织之间无差异。两种丝抑蛋白的浓度均与TNM分期无关。丝抑蛋白A在鳞状细胞癌中的含量高于腺癌（p < 0.01），而丝抑蛋白B则无此差异。在研究原发性肿瘤患者的生存概率关系时发现，丝抑蛋白B浓度的增加以及总半胱氨酸蛋白酶抑制活性与生存概率呈正相关，而丝抑蛋白A浓度与生存概率无此相关性。结论是，丝抑蛋白A和B是原发性肺肿瘤中半胱氨酸蛋白酶抑制活性的主要贡献者。丝抑蛋白B被证明是一个预后因素，尤其是在鳞状细胞癌中。
