Daigo Y, Suzuki K, Maruyama O, Miyoshi Y, Yasuda T, Kabuto T, Imaoka S, Fujiwara T, Takahashi E, Fujino M A, Nakamura Y
Laboratory of Molecular Medicine, University of Tokyo, Japan.
Genes Chromosomes Cancer. 1997 Oct;20(2):204-7.
We have isolated a human cDNA encoding a 115-amino-acid polypeptide that revealed 97% identity to a candidate tumor suppressor gene for oral cancer in Mesocricetus auratus (deleted in oral cancer-1; doc-1). It also showed a high degree of homology to a gene induced by TNF-alpha in Mus musculus. To investigate its possible role in esophageal carcinogenesis, we examined genetic alterations and expression levels of the gene in 13 esophageal carcinoma cell lines and 10 primary esophageal carcinomas. No mutation nor reduction of expression was observed in any of the 23 cancer materials examined. These results imply that the human doc-1 homologue is unlikely to play a significant role in esophageal carcinogenesis, although its role in the TNF-alpha signaling pathway remains unclear. We mapped DOC1 to chromosome band 12q24.31 by fluorescence in situ hybridization.
我们分离出了一个编码115个氨基酸多肽的人类cDNA，该多肽与金黄地鼠口腔癌候选肿瘤抑制基因（口腔癌缺失基因-1；doc-1）有97%的同一性。它还与小家鼠中由肿瘤坏死因子-α诱导的一个基因有高度同源性。为了研究其在食管癌发生中的可能作用，我们检测了该基因在13个食管癌细胞系和10例原发性食管癌中的基因改变和表达水平。在所检测的23份癌组织材料中均未观察到突变或表达降低。这些结果表明，人类doc-1同源物在食管癌发生中不太可能起重要作用，尽管其在肿瘤坏死因子-α信号通路中的作用仍不清楚。我们通过荧光原位杂交将DOC1定位于染色体12q24.31带。
