Nakajima O, Hachisuka A, Takagi K, Yamazaki T, Ikebuchi H, Sawada J
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Tokyo, Japan.
Neuroreport. 1997 Sep 29;8(14):3005-8. doi: 10.1097/00001756-199709290-00003.
Opioid-binding cell adhesion molecule (OBCAM), neurotrimin (NTM) and limbic system-associated membrane protein (LAMP) are homologous and are the members of the IgLON family which is a subfamily within the immunoglobulin superfamily. We cloned the cDNAs for OBCAM and NTM, prepared recombinant proteins, and examined the reactivity of the previously prepared monocolonal anti-OBCAM antibody, OBC53, with the recombinant proteins by immunoblotting. These experiments revealed that OBC53 recognizes OBCAM about 1000 times as efficiently as NTM. Moreover, the NTM and LAMP peptides which have sequences homologous to the OBCAM peptide used for the preparation of OBC53 were 150 times less reactive to OBC53. Thus, the OBC53 antibody is a useful tool for specifically detecting OBCAM in immunochemical experiments.
阿片样物质结合细胞粘附分子（OBCAM）、神经调节素（NTM）和边缘系统相关膜蛋白（LAMP）是同源的，它们是免疫球蛋白超家族中的一个亚家族——IgLON家族的成员。我们克隆了OBCAM和NTM的cDNA，制备了重组蛋白，并通过免疫印迹检测先前制备的抗OBCAM单克隆抗体OBC53与重组蛋白的反应性。这些实验表明，OBC53识别OBCAM的效率约为NTM的1000倍。此外，与用于制备OBC53的OBCAM肽具有同源序列的NTM和LAMP肽对OBC53的反应性低150倍。因此，OBC53抗体是免疫化学实验中特异性检测OBCAM的有用工具。
