芽孢杆菌属FTU菌株的鉴定及caa3型氧化酶同源性研究。

Identification of Bacillus sp. FTU strain and the study of the caa3-type oxidase homology.

作者信息

Grinkevich V A, Lysenko A M, Muntyan M S, Skripnikova E V, Afrikyan E K

机构信息

School of Biology, Lomonosov Moscow State University, Russia.

出版信息

Biochemistry (Mosc). 1997 Jul;62(7):718-24.

PMID:9331963

Abstract

The culture, morphology, and genome of alkalo- and halotolerant bacterial strain Bacillus sp. FTU were characterized; the strain is compared to other representatives of genus Bacillus. The DNA-DNA hybridization data indicate that the strains of Bacillus halodurans DSM 497 and DSM 2513 and Bacillus sp. FTU belong to the same species. Bacillus sp. FTU can be renamed to Bacillus halodurans FTU. The N-terminal amino acid fragments of the subunits I and II of the terminal caa3-type cytochrome c oxidase of B. halodurans FTU were sequenced. The N-terminal fragments of this enzyme and of the caa3-type oxidase of alkalophilic Bacillus firmus OF4 are highly homologous (homology of subunits I and II is over 90 and over 96%, respectively). Such high homology of the terminal oxidases of these bacteria might be due to their alkaline medium.

摘要

对耐碱和耐盐细菌菌株芽孢杆菌属FTU的培养特性、形态学和基因组进行了表征；并将该菌株与芽孢杆菌属的其他代表菌株进行了比较。DNA-DNA杂交数据表明，嗜碱芽孢杆菌DSM 497和DSM 2513菌株以及芽孢杆菌属FTU属于同一物种。芽孢杆菌属FTU可重新命名为嗜碱芽孢杆菌FTU。对嗜碱芽孢杆菌FTU末端caa3型细胞色素c氧化酶亚基I和II的N端氨基酸片段进行了测序。该酶与嗜碱芽孢杆菌OF4的caa3型氧化酶的N端片段高度同源（亚基I和II的同源性分别超过90%和96%）。这些细菌末端氧化酶的高度同源性可能归因于它们所处的碱性环境。