一项关于从不同年龄段大鼠脑区获得的粗突触体组分中含3H-L-葡萄糖糖蛋白的研究。

A study of 3H-L-flucose-containing glycoproteins of the crude synaptosomal fraction obtained from rat brain regions at various ages.

作者信息

Davis L G, Brunngraber E G, Routtenberg A

出版信息

J Neurosci Res. 1976;2(1):83-8. doi: 10.1002/jnr.490020110.

DOI:10.1002/jnr.490020110

Abstract

The frontal cortex showed a marked elevation of incorporation of 3H-L-fucose into glycoproteins of the crude synaptosomal fraction at 9 days of age. The effect was observed at both 8 and 24 hr after intraperitoneal administration of radioactive fucose. The elevation was absent in the 1, 5, 16, and 25-day-old frontal cortices. It was also absent in the hippocampus and cerebellum at all ages studied. Fucose-labeled glycoproteins of high molecular weight in the crude synaptosomal fraction, as separated by polyacrylamide gels,were preferentially labeled in the hippocampus, frontal cortex, and cerebellum at all ages studied. In general, these glycoproteins became less prominent in the oldest animals studied (25 days). However, the relative amount of these glycoproteins varied with age of the animal, time between administration of isotope and sacrifice, and with the brain areas studied.

摘要

在9日龄时，额叶皮质中粗制突触体组分糖蛋白的3H-L-岩藻糖掺入量显著升高。在腹腔注射放射性岩藻糖后8小时和24小时均观察到这种效应。在1日龄、5日龄、16日龄和25日龄的额叶皮质中未出现这种升高。在所研究的所有年龄段，海马体和小脑中也未出现这种升高。通过聚丙烯酰胺凝胶分离的粗制突触体组分中高分子量的岩藻糖标记糖蛋白，在所有研究年龄段的海马体、额叶皮质和小脑中均被优先标记。一般来说，在研究的最年长动物（25日龄）中，这些糖蛋白变得不那么突出。然而，这些糖蛋白的相对含量随动物年龄、同位素注射与处死之间的时间以及所研究的脑区而变化。