Kudo T, Sutou S
Central Research Institute, Itoham Foods Inc., Ibaraki, Japan.
Gene. 1997 Sep 15;197(1-2):261-8. doi: 10.1016/s0378-1119(97)00270-9.
FTZ-F1 is a member of the orphan nuclear receptors, which belongs to the steroid hormone receptor superfamily, and plays a role in the blastoderm and nervous system development in Drosophila. Recently, several FTZ-F1 family genes have been cloned in several species. SF-1/Ad4BPs have been identified as master regulators controlling steroidogenic P-450 genes in mammals and are considered to be the mammalian homologues of FTZ-F1. Moreover, SF-1/Ad4BP plays a critical role in the sexual differentiation of gonads in mammals. In vertebrates, except for mammals, the functional homologue of SF-1/Ad4BP has not been identified before. Herein, we cloned two chicken cDNAs (OR2.0 and OR2.1), which encode putative FTZ-F1 family receptors, by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). OR2.1 consists of 3255 bp, is expressed in the adrenal glands and gonads, and is considered to be the chicken counterpart of mammalian SF-1/Ad4BP. However, OR2.0 consists of 2945 bp, is expressed in the livers and the adrenal glands, and is considered to be the chicken counterpart of mouse LRH-1, which is a member of the FTZ-F1 family in mammals.
FTZ - F1是孤儿核受体家族的成员，属于类固醇激素受体超家族，在果蝇的胚盘和神经系统发育中发挥作用。最近，在多个物种中克隆出了几个FTZ - F1家族基因。SF - 1/Ad4BPs已被确定为哺乳动物中控制类固醇生成性P - 450基因的主要调节因子，并被认为是FTZ - F1的哺乳动物同源物。此外，SF - 1/Ad4BP在哺乳动物性腺的性别分化中起关键作用。在脊椎动物中，除了哺乳动物外，此前尚未鉴定出SF - 1/Ad4BP的功能同源物。在此，我们通过逆转录聚合酶链反应（RT - PCR）和cDNA末端快速扩增（RACE）克隆了两个鸡的cDNA（OR2.0和OR2.1），它们编码推定的FTZ - F1家族受体。OR2.1由3255个碱基对组成，在肾上腺和性腺中表达，被认为是哺乳动物SF - 1/Ad4BP的鸡对应物。然而，OR2.0由2945个碱基对组成，在肝脏和肾上腺中表达，被认为是小鼠LRH - 1的鸡对应物，小鼠LRH - 1是哺乳动物FTZ - F1家族的成员。
