Lavania U C, Sharma A K
Stain Technol. 1979 Sep;54(5):261-4. doi: 10.3109/10520297909110682.
A convenient and quick method using trypsin-orcein for banding plant chromosomes (O-banding) is suggested. The technique is directly applicable to meristematic tissues (e.g. root tips) and involves the treatment of root tips with 1-2% solution of trypsin either in buffer or in 0.5 N HCl for 5-10 minutes at 37 C or for 30-60 minutes near 0 C followed by staining with 1.5% acetic orcein: 1 N HCl (19:1). Dark staining bands are reproducible and species specific. These bands possibly represent specific DNA-protein-dye interaction.
本文提出了一种使用胰蛋白酶-地衣红进行植物染色体显带（O带）的简便快速方法。该技术可直接应用于分生组织（如根尖），具体操作是将根尖用1%-2%的胰蛋白酶溶液处理，该溶液可置于缓冲液中，也可置于0.5N盐酸中，在37℃下处理5-10分钟，或在接近0℃的温度下处理30-60分钟，随后用1.5%的醋酸地衣红：1N盐酸（19:1）进行染色。深色染色带具有可重复性且具有物种特异性。这些带可能代表特定的DNA-蛋白质-染料相互作用。
