Valaer A K, Huber T, Andurkar S V, Clark C R, DeRuiter J
Alabama Reference Laboratory, Montgomery 36103, USA.
J Chromatogr Sci. 1997 Oct;35(10):461-6. doi: 10.1093/chromsci/35.10.461.
A sensitive, specific urinary assay for fentanyl, sufentanil, and alfentanil based on their N-dealkylated metabolites is described. Norfentanyl, norsufentanil-noralfentanil, and 2H5-norfentanyl are synthesized and characterized by standard analytical techniques. Derivatization of these secondary amines to yield the pentafluorobenzamides produces stable products with good gas chromatographic properties and unique, high-mass fragments in their mass spectra. These properties are utilized to develop a drug screening procedure based on gas chromatography-mass spectrometry to detect these major metabolites in human urine. The metabolites are isolated from urine samples by a liquid-liquid extraction procedure. The method allows for detection of metabolite concentrations as low as 0.3 ng/mL.
本文描述了一种基于芬太尼、舒芬太尼和阿芬太尼的N-去烷基化代谢物的灵敏、特异的尿液检测方法。合成了去甲芬太尼、去甲舒芬太尼-去甲阿芬太尼和2H5-去甲芬太尼，并采用标准分析技术对其进行了表征。将这些仲胺衍生化生成五氟苯甲酰胺，得到具有良好气相色谱性质的稳定产物，且其质谱图中有独特的高质量碎片。利用这些性质开发了一种基于气相色谱-质谱联用的药物筛查程序，以检测人尿中的这些主要代谢物。通过液-液萃取程序从尿液样本中分离代谢物。该方法能够检测低至0.3 ng/mL的代谢物浓度。
