Babakhin A A, Majoul L A, Vedernikov A A, Leskov V P, Pisarev V M, Babakhin A A, Gushchin I S, Nolte H, DuBuske L M
National Research Center, Institute of Immunology, Moscow, Russia.
Allergy Asthma Proc. 1997 Sep-Oct;18(5):301-10. doi: 10.2500/108854197778590470.
The mycelial mass of the fungus Polyporus Squamosus strain 64 (PS-64) was disintegrated by mechanical and ultrasound treatments. After centrifugation, the supernatant containing the disintegrate was dialyzed and lyophilized. The resultant PS-64 extract was subsequently investigated as an immunomodulator of IgE and IgG responses to ovalbumin (OA) in (CBAxC57BL/6)F1 mice using passive cutaneous anaphylaxis (PCA) and enzyme-linked immunosorbent assay (ELISA), respectively. Multiple injections of PS-64 extract in doses of 1.5, 15, and 150 mg/kg administered before the primary or secondary immunization of mice with OA resulted in a dose-dependent inhibition of both IgE and IgG antibody responses to OA. In contrast to the inhibition of the anti-OA IgE response noted during the entire 3-week observation period, the anti-OA IgG response was restored to control level by the third week of secondary immunization. The glass microfiber-based whole blood histamine release assay demonstrated that various concentrations of the PS-64 extract did not influence histamine release induced either by anti-IgE or by specific allergens from basophils derived from whole blood of allergen-sensitized patients. Using the hemolytic plaque assay, significant suppression of IgM-secreting cell formation was noted in (CBAxC57BL/6)F1 mice administered various doses of the PS-64 extract before immunization. The PS-64 extract inhibited the in vitro proliferation of human mononuclear cells upon stimulation with phytohemagglutinin (PHA). In a dose-dependent manner, the PS-64 extract also inhibited delayed-type hypersensitivity reaction and skin graft rejection, similar to the effect noted with usage of Cyclosporin A (CsA) in (CBAxC57BL/6)F1 mice. Our investigation suggests that the immunomodulatory effects of PS-64 should be studied further for potential clinical therapeutic utility.
真菌鳞多孔菌菌株64（PS - 64）的菌丝体通过机械和超声处理进行分解。离心后，将含有分解物的上清液进行透析并冻干。随后，使用被动皮肤过敏反应（PCA）和酶联免疫吸附测定（ELISA），分别研究所得的PS - 64提取物作为（CBAxC57BL/6）F1小鼠中针对卵清蛋白（OA）的IgE和IgG反应的免疫调节剂。在用OA对小鼠进行初次或二次免疫之前，多次注射剂量为1.5、15和150 mg/kg的PS - 64提取物，导致对OA的IgE和IgG抗体反应均出现剂量依赖性抑制。与在整个3周观察期内观察到的抗OA IgE反应受到抑制不同，二次免疫的第三周时，抗OA IgG反应恢复到对照水平。基于玻璃微纤维的全血组胺释放试验表明，不同浓度的PS - 64提取物不会影响由抗IgE或来自变应原致敏患者全血的嗜碱性粒细胞的特异性变应原诱导的组胺释放。使用溶血空斑试验，在免疫前给予不同剂量PS - 64提取物的（CBAxC57BL/6）F1小鼠中，观察到分泌IgM的细胞形成受到显著抑制。PS - 64提取物在受到植物血凝素（PHA）刺激后抑制人单核细胞的体外增殖。PS - 64提取物还以剂量依赖性方式抑制迟发型超敏反应和皮肤移植排斥反应，这与在（CBAxC57BL/6）F1小鼠中使用环孢素A（CsA）时观察到的效果相似。我们的研究表明，应进一步研究PS - 64的免疫调节作用，以探讨其潜在的临床治疗用途。
