Coscarella A, Carloni C, Liddi R, Mauro S, Novelli S, Mele A, Masella B, Valtieri M, De Santis R
Menarini Ricerche SpA, Biotechnology Research Department, Rome, Italy.
Eur J Haematol. 1997 Oct;59(4):238-46. doi: 10.1111/j.1600-0609.1997.tb00983.x.
Selective lineage differentiation depends upon the combined action of several colony-stimulating factors. Here we describe 3 human granulocyte-macrophage colony-stimulating factor-erythropoietin (GM-CSF-EPO) hybrid proteins generated by recombination of the relevant cDNAs. The expression vector containing the murine cytomegalovirus (mCMV) promoter and dihydrofolate reductase (DHFR) gene was used for the expression of the hybrid genes in Chinese hamster ovary (CHO) cells. Purified hybrid proteins from CHO transfectant cultures induced proliferation of both EPO and GM-CSF dependent cell lines. The clonogenic test, performed on purified human hematopoietic precursor cells, indicates that the hybrid proteins are more efficient at inducing erythroid differentiation compared with the equimolar mixture of GM-CSF and EPO.
选择性谱系分化取决于几种集落刺激因子的联合作用。在此，我们描述了通过相关cDNA重组产生的3种人粒细胞-巨噬细胞集落刺激因子-促红细胞生成素（GM-CSF-EPO）杂合蛋白。含有鼠巨细胞病毒（mCMV）启动子和二氢叶酸还原酶（DHFR）基因的表达载体用于在中国仓鼠卵巢（CHO）细胞中表达杂合基因。从CHO转染细胞培养物中纯化的杂合蛋白可诱导EPO和GM-CSF依赖性细胞系的增殖。对纯化的人造血前体细胞进行的克隆形成试验表明，与GM-CSF和EPO的等摩尔混合物相比，杂合蛋白在诱导红系分化方面更有效。
