Muñoz G, Nakari-Setälä T, Agosin E, Penttilä M
Departamento de Ingeniería Química y Bioprocesos, Pontificia Universidad Católica de Chile, Santiago, Chile.
Curr Genet. 1997 Sep;32(3):225-30. doi: 10.1007/s002940050270.
A cDNA clone encoding a spore-related hydrophobin, SRHI, was isolated from a cDNA bank prepared from mRNA induced in sporulating cultures of Trichoderma harzianum by heterologous hybridization using the hfb2 gene encoding a spore-bound hydrophobin of Trichoderma reesei as a probe. Based on sequence similarity the predicted protein was identified as a new member of the class-II hydrophobin family. Including the signal sequences, SRHI has 65% and 56% amino-acid similarity with the T. reesei hydrophobins HFBII and HFBI, respectively, being less similar with other hydrophobins. srh1 is present as one copy in the T. harzianum genome. It is highly expressed under sporulating conditions, both in submerged as well as in aerial cultures. Moreover, nutrient limitation induces srh1 expression.
通过使用编码里氏木霉孢子结合疏水蛋白的hfb2基因作为探针，从哈茨木霉孢子形成培养物中诱导的mRNA制备的cDNA文库中，分离出一个编码孢子相关疏水蛋白SRHI的cDNA克隆。基于序列相似性，预测的蛋白质被鉴定为II类疏水蛋白家族的一个新成员。包括信号序列，SRHI与里氏木霉疏水蛋白HFBII和HFBI的氨基酸相似性分别为65%和56%，与其他疏水蛋白的相似性较低。srh1在哈茨木霉基因组中以单拷贝形式存在。它在孢子形成条件下高度表达，无论是在液体培养还是在固体培养中。此外，营养限制会诱导srh1表达。
