Hägg P M, Horelli-Kuitunen N, Eklund L, Palotie A, Pihlajaniemi T
Collagen Research Unit, University of Oulu, Finland.
Genomics. 1997 Oct 1;45(1):31-41. doi: 10.1006/geno.1997.4884.
We report on full-length mouse type XV collagen cDNAs that encode a 1367-residue alpha 1(XV) chain. The amino acid sequences of the mouse and previously characterized human alpha 1(XV) chains exhibit an overall identity of 72%. The highest homology between these chains and to the structurally related type XVIII collagen is observed in their C-terminal noncollagenous domains. Although the mouse and human alpha 1(XV) chains are highly homologous and similar in their overall domain structure, the mouse chain contains only seven collagenous domains, whereas the human chain contains nine. Northern analysis of several mouse tissues indicated strong hybridization in the case of heart and skeletal muscle RNAs and moderate signals with kidney, lung, and testis RNAs. Analysis of type XV collagen mRNA levels at different stages of mouse embryonic development indicated a marked increase in the level between 11 and 15 days of development, which coincides with pronounced development of the muscles, heart, and vascular system in the mouse embryo. The mouse gene for type XV collagen was mapped by fluorescence in situ hybridization to chromosome 4, band B1-3. This result indicates that the mouse type XV collagen gene and its human counterpart are located in the chromosomal segments with conserved syntenies.
我们报道了编码1367个残基的α1(XV)链的全长小鼠XV型胶原蛋白cDNA。小鼠α1(XV)链与人α1(XV)链（此前已鉴定）的氨基酸序列总体一致性为72%。这些链与结构相关的XVIII型胶原蛋白之间的最高同源性出现在它们的C端非胶原结构域。尽管小鼠和人α1(XV)链高度同源且总体结构域相似，但小鼠链仅包含7个胶原结构域，而人链包含9个。对几种小鼠组织的Northern分析表明，心脏和骨骼肌RNA有强杂交信号，肾脏、肺和睾丸RNA有中等信号。对小鼠胚胎发育不同阶段的XV型胶原蛋白mRNA水平分析表明，在发育的11至15天之间水平显著增加，这与小鼠胚胎中肌肉、心脏和血管系统的明显发育相吻合。通过荧光原位杂交将小鼠XV型胶原蛋白基因定位到4号染色体B1-3带。这一结果表明，小鼠XV型胶原蛋白基因及其人类对应基因位于具有保守同线性的染色体区段。
