Marazziti D, Golini E, Gallo A, Lombardi M S, Matteoni R, Tocchini-Valentini G P
Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Rome, Italy.
Genomics. 1997 Oct 1;45(1):68-77. doi: 10.1006/geno.1997.4900.
A cDNA sequence encoding a putative peptide-specific G-protein-coupled receptor (GPR37) was isolated from a set of human brain frontal lobe expressed sequence tags. The GPR37 cDNA predicts a single open reading frame coding for a 613-amino-acid protein with seven hydrophobic transmembrane domains. The GPR37 genomic sequence was mapped to chromosome 7q31, and it was isolated upon screening of a chromosome 7-specific genomic library. The GPR37 gene spans more than 25 kb and contains two exons and a single intron which interrupts the GPR37 cDNA within the sequence encoding the presumed third transmembrane domain. Northern blot analysis with GPR37 probes revealed a main 3.8-kb mRNA and a less abundant 8-kb mRNA, both expressed in human brain tissues, particularly in corpus callosum, medulla, putamen, and caudate nucleus. The lowest level of expression was detected in cerebellum. The 3.8-kb mRNA is also less abundantly expressed in liver and placenta. Although the ligand for the putative GPR37 receptor has not been identified, its deduced amino acid sequence shows a high degree of homology (approximately 40% in the transmembrane regions) with most mammalian peptide-specific G-protein-coupled receptors and particularly with the human endothelin-B, bombesin-BB1, and bombesin-BB2 receptors.
从一组人脑海马区表达序列标签中分离出一个编码假定的肽特异性G蛋白偶联受体（GPR37）的cDNA序列。GPR37 cDNA预测有一个单一的开放阅读框，编码一个613个氨基酸的蛋白质，该蛋白质具有七个疏水跨膜结构域。GPR37基因组序列被定位到7号染色体q31区，通过筛选7号染色体特异性基因组文库将其分离出来。GPR37基因跨度超过25kb，包含两个外显子和一个单一内含子，该内含子在编码假定的第三个跨膜结构域的序列内中断GPR37 cDNA。用GPR37探针进行的Northern印迹分析显示，主要有一个3.8kb的mRNA和一个丰度较低的8kb的mRNA，二者均在人脑组织中表达，尤其是在胼胝体、延髓、壳核和尾状核中表达。在小脑中检测到的表达水平最低。3.8kb的mRNA在肝脏和胎盘中的表达量也较低。虽然尚未鉴定出假定的GPR37受体的配体，但其推导的氨基酸序列与大多数哺乳动物肽特异性G蛋白偶联受体显示出高度同源性（跨膜区域约为40%），特别是与人内皮素-B、蛙皮素-BB1和蛙皮素-BB2受体。 （注：原文中是额叶，译文里前面误写为海马区，按照正确的额叶翻译了）
