Schofield J P, Elgar G, Greystrong J, Lye G, Deadman R, Micklem G, King A, Brenner S, Vaudin M
Department of Medicine, University of Cambridge, Addenbrooke's Hospital, United Kingdom.
Genomics. 1997 Oct 1;45(1):158-67. doi: 10.1006/geno.1997.4913.
We have isolated and sequenced a cosmid clone from the compact genome of the Japanese pufferfish (Fugu rubripes) containing portions of three genes that have the same order as in human. The gene order is microtubule-associated protein (MAP-2), myosin light chain (MYL-1), and carbamoyl phosphate synthetase (CPS III). The intron-exon organization of Fugu CPS III is identical with that of rat CPS I, although the equivalent genomic fragments of rat and Fugu CPS span 87.9 and 21 kb, respectively. This is the first report of a piscine CPS III genomic structure and predicts a close evolutionary link between CPS III and CPS I. The 8-kb intergenic region between MYL-1 and CPS gave no clear areas of transcription factor-binding sites by pairwise comparison with shark or rat CPS promoter regions. However, there was a match with the rat myosin light chain 2 (MLC-2) gene promoter and a MyoD transcription factor-binding site 874 bp upstream of the MYL-1 gene.
我们从日本河豚（红鳍东方鲀）紧凑的基因组中分离并测序了一个黏粒克隆，该克隆包含三个基因的部分片段，其排列顺序与人类相同。基因顺序为微管相关蛋白（MAP - 2）、肌球蛋白轻链（MYL - 1）和氨甲酰磷酸合成酶（CPS III）。河豚CPS III的内含子 - 外显子结构与大鼠CPS I相同，尽管大鼠和河豚CPS的等效基因组片段分别跨越87.9 kb和21 kb。这是关于鱼类CPS III基因组结构的首次报道，并预测CPS III与CPS I之间存在密切的进化联系。通过与鲨鱼或大鼠CPS启动子区域进行成对比较，MYL - 1和CPS之间8 kb的基因间区域未发现明显的转录因子结合位点区域。然而，在大鼠肌球蛋白轻链2（MLC - 2）基因启动子以及MYL - 1基因上游874 bp处存在一个肌分化因子（MyoD）转录因子结合位点。
