Bomhoff G, Klapwijk P M, Kester H C, Schilperoort R A, Hernalsteens J P, Schell J
Mol Gen Genet. 1976 May 7;145(2):177-81. doi: 10.1007/BF00269591.
Several nopaline degrading strains and one octopine degrading strain are shown to loose oncogenicity as well as the ability to utilize these guanidine compounds when they are cured of their TI plasmid. To investigate whether the specific genes involved in the utilization of one or the other compound are located on the plasmid, plasmid-transfer experiments have been performed. The plasmid from a nopaline degrading strain has been transferred to a naturally non oncogenic Agrobacterium namely A. radiobacter. Furthermore, the plasmid from an octopine degrading strain has been transferred to a plasmid-cured strain which originally had the capacity to utilize nopaline. Both kinds of experiments prove that the TI plasmid determines the strain specificity with regard to the utilization of either octopine or nopaline. They also demonstrate that the synthesis of either octopine or nopaline in crown gall cells is also determined by genes located on the TI plasmid harboured by the transforming A. tumefaciens strains.
几种胭脂碱降解菌株和一种章鱼碱降解菌株在被消除Ti质粒后，显示出丧失致癌性以及利用这些胍类化合物的能力。为了研究参与利用这一种或另一种化合物的特定基因是否位于质粒上，已进行了质粒转移实验。来自一种胭脂碱降解菌株的质粒已被转移到一种天然无致癌性的土壤杆菌，即放射土壤杆菌。此外，来自一种章鱼碱降解菌株的质粒已被转移到一个原来有能力利用胭脂碱的质粒消除菌株。这两类实验都证明，Ti质粒决定了菌株在利用章鱼碱或胭脂碱方面的特异性。它们还表明，冠瘿瘤细胞中章鱼碱或胭脂碱的合成也由转化的根癌土壤杆菌菌株所携带的Ti质粒上的基因决定。
