nm23-H1和nm23-H2基因座上新微卫星的分离、特征分析及其在杂合性缺失（LOH）分析中的应用。

Isolation and characterization of new microsatellites at the nm23-H1 and nm23-H2 gene loci and application for loss of heterozygosity (LOH) analysis.

作者信息

Seifert M, Theisinger B, Engel M, Seib T, Seitz G, Stolte M, Hilgert K, Welter C

机构信息

Institut für Humangenetik, Universität des Saarlandes, Homburg/Saar, Germany.

出版信息

Hum Genet. 1997 Oct;100(5-6):515-9. doi: 10.1007/s004390050545.

DOI:10.1007/s004390050545

PMID:9341864

Abstract

The nm23-H1 gene has been suggested to be a metastasis suppressor gene. Studies about the events of loss of heterozygosity (LOH) at the nm23 locus and its correlation to metastasis are controversially discussed. To optimize detection of LOH at the nm23 locus, we screened two P1 clones for additional microsatellites. Tumor and normal DNA from 37 colorectal, 16 gastric, and 8 germ cancer patients were examined for LOH. We found two new CA repeats, one 5' to nm23-H1 and another 3' to nm23-H2. Using these nm23 locus-specific CA repeats and five other chromosome 17 loci (D17S1522, D17S1566, D17S855, D17S515, and TP53), allele loss was observed in 4/32 (12.5%) patients with colon cancer, 2/14 (14.3%) with gastric cancer, and 1/7 (14%) with germ cancer. No isolated LOH of the nm23 region was observed.

摘要

nm23-H1基因被认为是一种转移抑制基因。关于nm23基因座杂合性缺失（LOH）事件及其与转移的相关性研究存在争议。为了优化nm23基因座LOH的检测，我们筛选了两个P1克隆以寻找额外的微卫星。检测了37例结直肠癌、16例胃癌和8例生殖细胞癌患者的肿瘤和正常DNA中的LOH。我们发现了两个新的CA重复序列，一个在nm23-H1上游5'处，另一个在nm23-H2下游3'处。使用这些nm23基因座特异性CA重复序列和其他5个17号染色体基因座（D17S1522、D17S1566、D17S855、D17S515和TP53），在32例结肠癌患者中有4例（12.5%）、14例胃癌患者中有2例（14.3%）、7例生殖细胞癌患者中有1例（14%）观察到等位基因缺失。未观察到nm23区域的孤立性LOH。