Bai X, Hosler G, Rogers B B, Dawson D B, Scheuermann R H
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235-9072, USA.
Clin Chem. 1997 Oct;43(10):1843-9.
Human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. We report the development and validation of a quantitative PCR method to measure viral burden for all eight human herpesviruses (HSV1, HSV2, VZV, EBV, CMV, HHV6, HHV7, and KSHV) in patients' samples. The method uses an internal standard that is coamplified with the viral target, allowing quantification of viral genomes in absolute terms (e.g., viral targets/mL of blood) and ruling out false-negative results. We demonstrate that transplant patients with lymphoproliferative disorder carry an EBV viral burden 3 logs higher than nontransplant patients. EBV titers in transplant patients without a lymphoproliferative disorder are between these values. This quantitative PCR method may aid in differentiating clinically significant vs latent viral burden in immunosuppressed patients.
人类疱疹病毒可引发水痘或单核细胞增多症等急性疾病，也可能在免疫抑制期间重新激活，导致带状疱疹或淋巴增殖性疾病等严重或危及生命的疾病。我们报告了一种定量PCR方法的开发与验证，该方法用于测量患者样本中所有八种人类疱疹病毒（HSV1、HSV2、VZV、EBV、CMV、HHV6、HHV7和KSHV）的病毒载量。该方法使用与病毒靶标共同扩增的内标，从而能够绝对定量病毒基因组（例如，每毫升血液中的病毒靶标数）并排除假阴性结果。我们证明，患有淋巴增殖性疾病的移植患者携带的EBV病毒载量比非移植患者高3个对数。没有淋巴增殖性疾病的移植患者的EBV滴度介于两者之间。这种定量PCR方法可能有助于区分免疫抑制患者中具有临床意义的病毒载量与潜伏性病毒载量。
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