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Errors in published sequences of human cytomegalovirus primers and probes: do we need more quality control?

作者信息

Habbal Wafa, Monem Fawza, Gärtner Barbara Christine

出版信息

J Clin Microbiol. 2005 Oct;43(10):5408-9. doi: 10.1128/JCM.43.10.5408-5409.2005.

DOI:10.1128/JCM.43.10.5408-5409.2005
PMID:16208034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1248504/
Abstract
摘要

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Cytomegalovirus infection in organ-transplant recipients: diagnostic value of pp65 antigen test, qualitative polymerase chain reaction (PCR) and quantitative Taqman PCR.器官移植受者的巨细胞病毒感染：pp65抗原检测、定性聚合酶链反应（PCR）及定量Taqman PCR的诊断价值
Transplantation. 2004 Jun 15;77(11):1692-8. doi: 10.1097/01.tp.0000133992.89191.52.
2
Identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and DNA real-time PCR detection.使用形态学、免疫组织化学和DNA实时聚合酶链反应检测在液基妇科样本中鉴定巨细胞病毒。
Diagn Cytopathol. 2004 Jun;30(6):411-7. doi: 10.1002/dc.20048.
3
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients.定性和定量聚合酶链反应在骨髓和肾移植受者巨细胞病毒感染诊断中的应用
Rev Soc Bras Med Trop. 2004 Mar-Apr;37(2):158-64. doi: 10.1590/s0037-86822004000200009. Epub 2004 Apr 13.
4
Quantitative real-time PCR with automated sample preparation for diagnosis and monitoring of cytomegalovirus infection in bone marrow transplant patients.采用自动样本制备的定量实时聚合酶链反应用于诊断和监测骨髓移植患者的巨细胞病毒感染
Clin Chem. 2004 May;50(5):846-56. doi: 10.1373/clinchem.2003.026484. Epub 2004 Mar 9.
5
PCR with the aqueous humor, blood leukocytes and vitreous of patients affected by cytomegalovirus retinitis and immune recovery uveitis.对受巨细胞病毒性视网膜炎和免疫恢复性葡萄膜炎影响的患者的房水、血液白细胞和玻璃体进行聚合酶链反应。
Ophthalmologica. 2004 Jan-Feb;218(1):43-8. doi: 10.1159/000074566.
6
Comparison of LightCycler-based PCR, COBAS amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation.基于LightCycler的聚合酶链反应、COBAS扩增仪巨细胞病毒监测仪和pp65抗原血症检测法在实体器官移植患者外周血标本中巨细胞病毒病毒载量定量测定的比较。
J Clin Microbiol. 2003 Jul;41(7):3167-74. doi: 10.1128/JCM.41.7.3167-3174.2003.
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A rapid monitoring system of human herpesviruses reactivation by LightCycler in stem cell transplantation.利用LightCycler在干细胞移植中对人类疱疹病毒再激活的快速监测系统
Bone Marrow Transplant. 2001 Nov;28(10):975-80. doi: 10.1038/sj.bmt.1703266.
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Detection of cytomegalovirus DNA in human specimens by LightCycler PCR: melting point analysis is mandatory to detect virus strains with point mutations in the target sequence of the hybridization probes.采用LightCycler PCR技术检测人标本中的巨细胞病毒DNA：对于检测杂交探针靶序列中存在点突变的病毒株，熔解温度分析必不可少。
J Clin Microbiol. 2001 Oct;39(10):3809. doi: 10.1128/JCM.39.10.3809.2001.