Arai I, Komatsu Y, Hirai Y, Shingu K, Ida Y, Yamaura H, Yamamoto T, Kuroiwa Y, Sasaki K, Taguchi S
Central Research Laboratories, Tsumura & Co., Ibaraki, Japan.
Planta Med. 1997 Oct;63(5):419-24. doi: 10.1055/s-2006-957725.
Our previous report stated that kikyo-to, a Japanese herbal medicine, consisting of the roots of Platycodon grandiflorum and Glycyrrhiza sp., stimulates the pancreatic exocrine secretion of conscious rats. The present study focused on the effective components of kikyo-to and the mechanism of stimuli to pancreatic secretion of rats. When 10 to 100 mg of platycodin D, a saponin from the root of Platycodon grandiflorum, was intragastrically administered, the pancreatic secretion of rats was stimulated. At the same time, the plasma CCK concentration increased. On the other hand, the stimulative effects of glycyrrhizin, a saponin from the root of Glycyrrhiza sp. were weak compared to platycodin D. The effects of 10 mg/kg of platycodin D on pancreatic secretion were inhibited by loxiglumide (50 mg/kg, i.g.), a CCK receptor antagonist. In contrast, the suppressive effect of atropine (300 micrograms/kg/h, i.v.) on pancreatic secretion was reduced by administering 10 mg/kg of platycodin D. In addition, up to 1 mM of platycodin D did not inhibit the trypsin activities in vitro. In conclusion, kikyo-to serves to stimulate pancreatic exocrine secretion mainly because platycodin D causes gastrointestinal hormones, particularly, CCK to be released from the duodenum.
我们之前的报告指出，桔梗汤（一种由桔梗根和甘草组成的日本草药）能刺激清醒大鼠的胰腺外分泌。本研究聚焦于桔梗汤的有效成分以及刺激大鼠胰腺分泌的机制。当胃内给予10至100毫克桔梗皂苷D（一种从桔梗根中提取的皂苷）时，大鼠的胰腺分泌受到刺激。同时，血浆CCK浓度升高。另一方面，甘草皂苷（一种从甘草根中提取的皂苷）的刺激作用与桔梗皂苷D相比较弱。10毫克/千克的桔梗皂苷D对胰腺分泌的作用被CCK受体拮抗剂洛莫司汀（50毫克/千克，胃内给药）抑制。相反，通过给予10毫克/千克的桔梗皂苷D可降低阿托品（300微克/千克/小时，静脉注射）对胰腺分泌的抑制作用。此外，高达1毫摩尔的桔梗皂苷D在体外并不抑制胰蛋白酶活性。总之，桔梗汤主要通过桔梗皂苷D促使十二指肠释放胃肠激素，特别是CCK，从而刺激胰腺外分泌。
