脂多糖（LPS）和CD40配体刺激对树突状细胞和巨噬细胞诱导产生白细胞介素-12的不同影响。

Differential effects of LPS and CD40 ligand stimulations on the induction of IL-12 production by dendritic cells and macrophages.

作者信息

Kato T, Yamane H, Nariuchi H

机构信息

The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan.

出版信息

Cell Immunol. 1997 Oct 10;181(1):59-67. doi: 10.1006/cimm.1997.1196.

DOI:10.1006/cimm.1997.1196

PMID:9344497

Abstract

In this experiment, we have examined the IL-12 production of murine macrophages (Mphi) and dendritic cells (DC) in response to LPS and CD40 ligand (CD40L) stimulations. Splenic Mphi and DC were purified by sorting on the basis of Mac-1 and CD11c expression and stimulated with LPS or Chinese hamster ovary cells expressing CD40L. The results showed that the ligation of CD40 induced the enhancement of IL-12 p40 mRNA accumulation and IL-12 production in DC as well as in Mphi; however, neither the accumulation of IL-12 p40 mRNA nor the production of bioactive IL-12 was detected in DC stimulated with various concentrations of LPS, although Mphi produced IL-12 on LPS stimulation. There was a remarkable difference in the expression of LPS receptor CD14 between Mphi and DC. Mphi evidently expressed CD14 but the CD14 expression of DC was quite low. Possible mechanisms of the failure in IL-12 production of DC are discussed.

摘要

在本实验中，我们检测了小鼠巨噬细胞（Mphi）和树突状细胞（DC）在脂多糖（LPS）和CD40配体（CD40L）刺激下白细胞介素-12（IL-12）的产生情况。通过基于Mac-1和CD11c表达进行分选来纯化脾Mphi和DC，并用LPS或表达CD40L的中国仓鼠卵巢细胞进行刺激。结果显示，CD40的结合诱导DC和Mphi中IL-12 p40 mRNA积累增强以及IL-12产生增加；然而，在用不同浓度LPS刺激的DC中，未检测到IL-12 p40 mRNA积累或生物活性IL-12的产生，尽管Mphi在LPS刺激下产生IL-12。Mphi和DC之间LPS受体CD14的表达存在显著差异。Mphi明显表达CD14，但DC的CD14表达相当低。本文讨论了DC无法产生IL-12的可能机制。