对包含MXI1和ADRA2A基因的150万个碱基对的酵母人工染色体（YAC）重叠群进行详细的物理分析。
Detailed physical analysis of a 1.5-megabase YAC contig containing the MXI1 and ADRA2A genes.
作者信息
Manca A, Volpi E V, Laficara F, Muresu R, Gray I C, Spurr N K, Nobile C
机构信息
Istituto di Genetica Molecolare del CNR, Alghero, Italy.
出版信息
Genomics. 1997 Oct 15;45(2):407-11. doi: 10.1006/geno.1997.4924.
The distal long arm of chromosome 10 harbors genes of biomedical interest such as MXI1, a putative tumor suppressor gene, and those encoding the adrenergic receptors alpha2A (ADRA2A) and beta1 (ADRB1). As part of a physical and genetic study of this genomic region, we constructed a 1.5-Mb YAC contig mapping to 10q25 that contains MXI1 and ADRA2A as well as a number of STSs. Rare cutting restriction site analysis of overlapping YACs allowed fine mapping of these genes and markers along the contig and revealed the presence of four CpG islands. MXI1 and ADRA2A appear to be about 600 kb apart, whereas ADRB1 is separated from ADRA2A by a distance larger than previously reported.
10号染色体的长臂远端含有一些具有生物医学研究价值的基因，如假定的肿瘤抑制基因MXI1，以及编码α2A肾上腺素能受体（ADRA2A）和β1肾上腺素能受体（ADRB1）的基因。作为对该基因组区域进行物理和遗传学研究的一部分，我们构建了一个定位到10q25的1.5兆碱基酵母人工染色体（YAC）重叠群，其中包含MXI1和ADRA2A以及一些序列标签位点（STS）。通过对重叠YAC进行稀有切割限制酶切位点分析，可对这些基因和标记物在重叠群上进行精细定位，并揭示了四个CpG岛的存在。MXI1和ADRA2A似乎相距约600千碱基，而ADRB1与ADRA2A之间的距离比先前报道的要大。
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