Bieganowski P, Bieganowska K, Zaborski J, Członkowska A
Maria Skłodowska-Curie Memorial Cancer Center, Wawelska, Poland.
Mult Scler. 1996 Sep;2(2):78-82. doi: 10.1177/135245859600200203.
We have used a PCR based method to analyse TCR gamma chain repertoire and clonality of gamma delta T cells in the CSF and blood of II MS patients. Samples collected from nine patients with other neurological diseases were used as a control. Five controls had central nervous system inflammation and four had non-inflammatory processes. We have observed a decreased percentage of gamma delta T cells expressing TCR gamma with V gamma 9 and J gamma P fragments in the CSF samples in comparison with the blood. We did not final clonal expansion of the gamma delta T cells in any control case. Clonal expansion of gamma delta T cells occurred in five of II MS cases in the CSF but not in the blood. Two of these clones expressed TCR gamma rearranged with V gamma 9 and J gamma 1 fragments, two others used V gamma 10 and J gamma P1, and one used V gamma 9 and J gamma P fragments. We found no correlation between clonality and clinical state of patients, duration of the disease or number of cells in CSF. Our study provides additional evidence for the possible role of the gamma delta T cells in the MS pathogenesis.
我们采用了一种基于聚合酶链反应（PCR）的方法，来分析11例多发性硬化症（MS）患者脑脊液和血液中γδT细胞的TCRγ链谱系及克隆性。从9例患有其他神经系统疾病的患者身上采集的样本用作对照。5名对照患者有中枢神经系统炎症，4名有非炎症性病变。我们观察到，与血液相比，脑脊液样本中表达带有Vγ9和JγP片段的TCRγ的γδT细胞百分比有所下降。在任何对照病例中，我们都未发现γδT细胞的最终克隆扩增。γδT细胞的克隆扩增发生在11例MS患者中的5例的脑脊液中，但未出现在血液中。其中两个克隆表达了与Vγ9和Jγ1片段重排的TCRγ，另外两个使用了Vγ10和JγP1，还有一个使用了Vγ9和JγP片段。我们发现克隆性与患者的临床状态、疾病持续时间或脑脊液中的细胞数量之间没有相关性。我们的研究为γδT细胞在MS发病机制中可能发挥的作用提供了更多证据。
