Morris R E, Ciraolo G M
Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, OH 45267, USA.
J Electron Microsc (Tokyo). 1997;46(4):315-9. doi: 10.1093/oxfordjournals.jmicro.a023525.
The authors have found that double etching of epoxy-embedded, ultrathin sections with 3% sodium metaperiodate rendered epitope expression comparable to that obtained with either saturated or half-saturated sodium metaperiodate solutions. In contrast to either of the two more concentrated oxidizing solutions, double etching with 3% sodium metaperiodate neither bleached the specimens nor generated holes in the plastic resin.
作者发现，用3%偏高碘酸钠对环氧树脂包埋的超薄切片进行双重蚀刻，其表位表达与用饱和或半饱和偏高碘酸钠溶液获得的表位表达相当。与两种浓度更高的氧化溶液中的任何一种不同，用3%偏高碘酸钠进行双重蚀刻既不会使标本褪色，也不会在塑料树脂中产生孔洞。
